MALDI-TOF MASS SPECTROMETER

生物化学系、化学系和生物系 布兰迪斯大学正在申请资助MALDI-TOF（矩阵- 辅助激光解吸电离飞行时间）质谱仪。 该仪器将被放置在核心生物分子合成和 分析设施。 它将免费提供给所有NIH支持的 布兰迪斯科学学院的研究人员，以及其他人， 有时间的基础上。 目前根本没有质谱仪 在布兰迪斯;目前，13名调查人员在主要用户 小组必须寻求校外合作进行任何质谱 任何品种的分析。 因此，购买最先进的 MALDI-TOF质谱仪将极大拓展生命科学 在这个机构的研究能力。提议的直接用途 主要用户群包括对产品的常规分析， 有机和生物有机合成（例如天然产物的合成， 修饰的核酸、功能化的蛋白质、酶抑制剂和 离子通道阻断剂）、翻译后修饰的定位位点 在蛋白质中，监测蛋白质磷酸化状态的变化， 新蛋白质和多肽的快速测序，同位素监测 用于NMR分析的蛋白质标记，监测金属离子置换 在金属蛋白质中，通过活性物质对蛋白质修饰位点进行定位， 酶定点灭活剂与肽动力学分析 通过蛋白酶的键断裂。 为获得和维持一个国家的体制承诺， 最先进的MALDI-TOF非常强大。 特别是，大学将 提供主要操作员的工资，以及保证 在可预见的将来维持该文书的资金。

Faculty in the Departments of Biochemistry, Chemistry and Biology at Brandeis University are requesting funding for a MALDI-TOF (Matrix- Assisted Laser Desorption Ionization Time-Of-Flight) mass spectrometer. This instrument will be placed in the Core Biomolecules Synthesis and Analysis Facility. It will be freely accessible to all NIH-supported researchers in the School of Science at Brandeis, as well as others on a time-available basis. Currently there is no mass spectrometer at all at Brandeis; at the present time the 13 investigators in the major user group must seek off-campus collaborations to carry out any mass spec analysis of any variety. Therefore acquisition of a state-of-the-art MALDI-TOF mass spectrometer will significantly expand the life science research capabilities at this institution. Immediate uses proposed by the major user group include routine analysis of the products from organic and bioorganic synthesis (e.g. synthesis of natural products, modified nucleic acids, functionalized proteins, enzyme inhibitors and ion channel blockers), mapping sites of post-translational modifications in proteins, monitoring changes in phosphorylation states of proteins, rapid sequencing of new proteins and peptides, monitoring isotope labeling of proteins for NMR analysis, monitoring metal ion replacement in metalloproteins, mapping sites of protein modification by active site-directed inactivators of enzymes and kinetic analysis of peptide bond cleavage by proteases. Institutional commitment for acquisition and maintenance of a state-of- the-art MALDI-TOF is very strong. In particular, the University will provide for the salary of the Principle Operator, as well as guarantee funds for maintenance of the instrument into the foreseeable future.