DEPARTMENTAL MASS SPECTROMETERS

这项提议支持购买两台质谱仪以供使用 16名生物医学研究人员，其中10名由美国国立卫生研究院资助，2名 开始成为教职员工。基质辅助激光解吸 电离飞行时间质谱仪和电喷雾 电离LC/MS/MS仪器将添加到已建立的核心质量中 化学与生物化学学院的光谱服务设施 在佐治亚理工学院和佐治亚州立大学， 在该地区有许多规模较小的学校。这个中心是对一个 成功建立了高分辨率共享核磁共振中心，位于 佐治亚州立大学。购买质谱仪的费用将是 佐治亚理工学院149,680美元和佐治亚理工学院16,000美元的匹配资金支持 佐治亚州立大学。质谱仪将用于 高分子量蛋白质和蛋白质的快速、相对分子质量测定 核酸，并将用于广泛的生化 分析。这些用户代表了一群高生产率的调查人员 对药物设计、结构生物化学和分子生物学感兴趣 生物学。用户组得到了几名教职员工的加强，网址为 佐治亚理工学院在几个领域进行尖端研究 质谱学的研究。设计中将使用质谱仪 具有重要治疗意义的新的合成蛋白酶抑制剂， 确定DNA是导体还是绝缘体，用于调查 硒在人体新陈代谢中的作用，决定了 白内障患者晶状体蛋白的变化，以及在设计 抗艾滋病毒和抗癌药物。

This proposal supports the acquisition of two mass spectrometers for use by 16 biomedical investigators of whom 10 are funded by the NIH and two are starting faculty members. A Matrix -assisted laser desorption ionization (MALDI) time-of-flight mass spectrometer and an electrospray ionization LC/MS/MS instrument will be added to an established core mass spectrometry service facility in the School of Chemistry and Biochemistry at the Georgia Institute of Technology and Georgia State University, along with numerous smaller schools in the region. This center complements a successful, established high resolution shared NMR center located at Georgia State University. The purchase of the mass spectrometers will be supported by matching funds of $149,680 from Georgia Tech and $16,000 from Georgia State University. The mass spectrometers will be used for the rapid, molecular mass determination of high molecular weight proteins and nucleic acids, and will be used for a broad spectrum of biochemical analyses. The users represent a highly productive group of investigators with interests in drug design, structural biochemistry, and molecular biology. The user group is strengthened by several faculty members at Georgia Tech who are performing cutting edge research in several fields of mass spectrometry. The mass spectrometers will be used in the design of new synthetic inhibitors for protease of therapeutic importance, for determining whether DNA is a conductor or an insulator, for investigating the role of selenium in human metabolism, determining the nature of the changes that occur in lens protein in cataracts, and in the design of anti-HIV and anticancer drugs.