LASER SCANNING CONFOCAL IMAGING SYSTEMS

本提案中要求的资金将用于建立激光 扫描共聚焦显微镜设备(LSCM)，位于 罗德岛医院的中心研究实验室。尼康PCM 2000系统将极大地扩展专业的研究能力 由六名NIH资助的调查人员组成的用户组，通过提供 尖端仪器和技术，将被用于 使用一系列对比模式研究活的和固定的细胞和组织 和荧光探头。多样化的组织阵列和分离/培养的 将分析细胞类型，包括肝、脑、脾、肠 粘膜和网状内皮系统的细胞(其中包括)。这个 尼康PCM 2000系统结合了：(A)尼康日食E 800研究 立式显微镜与尼康TE-200型倒置显微镜的研制 (配置亮场相位收缩、差分干扰 对比度和荧光显微镜光学)，(B)多波长 利用光纤耦合的Ar气体的激发能力 离子/HeNe激光光源；(C)多波长探测 通过高分辨率双通道荧光探测器系统；(D) 包括硬盘存档能力；(E)通过以太网传输数据 连接到邻近和/或远程预先存在的图像分析工作站；以及 (F)三维重建软件。提出的特定申请 这一手段可能包括：(A)检测和辨别 单标、双标或三标样品中的荧光探针； 对生命或pH敏感的细胞内活动的监测 荧光染料或与荧光标记相连的离子；和(C)光学 三维埋入试件的厚截面剖分 细胞和组织元素的重建。尼康PCM 2000系统 将满足不同生物医学群体的迫切需求 其研究计划将因 这一急需的仪器的可用性。

Funds requested in this proposal will be used to establish a Laser Scanning Confocal Microscope facility (LSCM) within the Division of Central Research Laboratories at Rhode Island Hospital. The Nikon PCM 2000 System will significantly extend the research capabilities of a major user group comprising of six NIH-funded investigators by providing cutting-edge instrumentation and technologies which will be utilized to study living and fixed cells and tissues using a range of contrast modes and fluorescent probes. A diverse array of tissues and isolated/cultured cell types will be analyzed including liver, brain, spleen, intestinal mucosa, and cells of the reticuloendothelial system (among others). The Nikon PCM 2000 System incorporates: (a) the Nikon Eclipse E 800 Research Upright Microscope and the Research Nikon TE-200 Inverted Microscope (configured with bright field phase contract, differential interference contrast, and fluorescence microscopy optics), (b) multiple wave length excitation capability by means of a fiber optically coupled, Argon Ion/HeNe laser illumination source; (c) multiple wave length detection through a high resolution, two channel fluorescence detector system; (d) including hard disk archiving capability; (e) data transfer via Ethernet to adjacent and/or remote pre-existing image analysis workstations; and (f) three dimensional reconstruction software. Specific applications made possible by this instrumentation include: (a) detection and discrimination of fluorescent probes in single-, double-, or triple-labeled samples; (b) monitoring of intracellular activities with vital or pH sensitive fluorescent dyes or ions conjugated to fluorescent tags; and (c) optical sectioning of thick sections of embedded specimens for three dimensional reconstruction of cellular and tissue elements. The Nikon PCM 2000 System will address the immediate needs of a diverse group of biomedical scientists whose research programs will benefit significantly by the availability of this much needed instrumentation.