Digitized Transmission Electron Microscope

描述(由申请人提供)：申请资金用于购买一台现代化的数码电子显微镜，安装在罗德岛医院(该州最大的布朗大学附属教学医院和创伤中心)的核心研究实验室部门内。摩根尼模型传输电子显微镜将大大有助于NIH资助的12名研究人员的研究和教学努力，他们的项目在本意见书中有详细介绍，以及布朗大学医学院的其他五家附属医院的医院教员的工作。我们目前有一台已有32年历史的飞利浦300透射电子显微镜。几个主要部件(例如高压电源、汞扩散真空泵、相机输送机构和至少两个镜头电源模块(又名K单元))在功能上不可靠，这些和/或其他元件似乎即将出现故障。我们仔细考虑了目前的透射式电子显微镜的技术能力和成本。价格范围“低端”的仪器足以满足我们的使用，具有出色的分辨率(相当于或非常接近高端型号)和相当大的灵活性，非常适合本提案中的研究项目，以及可能的未来项目。飞利浦(FEI)型号MOGANI 268是一台100千伏显微镜，配有6度倾斜和高分辨率数码相机系统的传统电动工作台。显微镜是完全数字化的，由一台可升级的PC控制，该PC也可用于数字图像捕获。这是飞利浦系列最近推出的型号，也是考虑过的最现代的型号。它具有非凡的价值；专为“细胞生物学、病理学、超微分子生物学”类别的用户而设计；非常适合我们的需求。与处于产品运行中期或接近尾声的模型不同，该单元处于其生产期的开始阶段。因此，显微镜将得到制造商的支持，服务和部件比其他产品更遥远，从而使其使用寿命更长。因此，由于功能、通用性、需求适当性、可升级性和整体价值，我们在此资金申请中指定了飞利浦摩根268。

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of a modern, digital, transmission electron microscope to be installed within the Division of Core Research Laboratories at Rhode Island Hospital (the state's largest Brown University-affiliated teaching hospital and trauma center). The Morgani Model Transmission Electron Microscope will significantly benefit the research and teaching endeavors of the twelve NIH-Funded investigators whose projects are detailed in this submission and that of Brown University campus-based investigators, as well the work of hospital-based faculty that are based at the five other Brown-affiliated hospitals which collectively comprise the Brown University School Of Medicine. We currently have a decrepit 32 year old Philips 300 transmission electron microscope. Several Major components (e.g., the high voltage supply, the mercury diffusion vacuum pump, the camera transport mechanism, and at least two lens supply modules {aka K units}) are functionally unreliable and frank failure of these and/or other elements appears imminent. We have carefully considered the technical capabilities and costs of current transmission electron microscopes. Instruments at the "low" end of their price ranges are adequate for our uses, give excellent resolution (equal to or very near high-end models) and considerable flexibility, and are well suited to the research projects in this proposal, as well as likely future projects. The Philips (FEI) model Morgani 268 is a 100 kV microscope with a conventional motorized stage with a 6 degree tilt & high-resolution digital camera system. The microscope is fully digitized, controlled by an upgradable PC that is also used for digital image capture. This is a recently introduced model in the Philips line, and is the most modern of those considered. It offers exceptional value; is designed for the "cell biology, pathology, ultrastructural molecular biology" category of users; and is well suited to our needs. Unlike models that are in the middle or near the end of their product runs, this unit is at the beginning of its production period. Consequently, the microscope will be supported by the manufacturer with service and parts further into the future than the others, giving it a longer useful life span. Therefore, due to capability, versatility, needs appropriateness, upgradability, and overall value, we have specified the Philips Morgani 268 in this funding request.