Digitized Transmission Electron Microscope

描述（由申请人提供）：罗德岛医院（该州最大的布朗大学附属教学医院和创伤中心）核心研究实验室部门需要购买一台现代化的数字透射电子显微镜。Morgani模型透射电子显微镜将极大地有利于12名美国国立卫生研究院资助的研究人员的研究和教学努力，他们的项目在本报告中有详细介绍，布朗大学校园研究人员的研究和教学工作，以及布朗大学医学院其他5家附属医院的医院教职员工的工作。我们目前有一个破旧的32岁的飞利浦300透射电子显微镜。几个主要部件（例如，高压电源，水银扩散真空泵，相机传输机构和至少两个镜头供应模块{aka K单元}）在功能上不可靠，这些和/或其他元件的直接故障迫在眉睫。我们仔细考虑了当前透射电子显微镜的技术能力和成本。仪器在其价格范围的“低”端是足够的，为我们的使用，提供优秀的分辨率（等于或非常接近高端机型）和相当大的灵活性，并非常适合本提案中的研究项目，以及可能的未来项目。该飞利浦（FEI）模型Morgani 268是一个100千伏显微镜与一个传统的6度倾斜和高分辨率数码相机系统的电动舞台。显微镜是完全数字化的，由一个可升级的个人电脑控制，也用于数字图像捕获。这是飞利浦系列中最近推出的型号，也是所考虑的最现代的型号。它提供了非凡的价值；是专为“细胞生物学、病理学、超微结构分子生物学”类用户设计的；而且非常适合我们的需要。与处于产品运行中期或接近尾声的模型不同，该单元处于生产周期的开始。因此，显微镜将由制造商提供服务和零件支持，比其他产品更深入未来，使其使用寿命更长。因此，由于能力、多功能性、需求适宜性、可升级性和整体价值，我们在本次资助申请中指定了飞利浦Morgani 268。

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of a modern, digital, transmission electron microscope to be installed within the Division of Core Research Laboratories at Rhode Island Hospital (the state's largest Brown University-affiliated teaching hospital and trauma center). The Morgani Model Transmission Electron Microscope will significantly benefit the research and teaching endeavors of the twelve NIH-Funded investigators whose projects are detailed in this submission and that of Brown University campus-based investigators, as well the work of hospital-based faculty that are based at the five other Brown-affiliated hospitals which collectively comprise the Brown University School Of Medicine. We currently have a decrepit 32 year old Philips 300 transmission electron microscope. Several Major components (e.g., the high voltage supply, the mercury diffusion vacuum pump, the camera transport mechanism, and at least two lens supply modules {aka K units}) are functionally unreliable and frank failure of these and/or other elements appears imminent. We have carefully considered the technical capabilities and costs of current transmission electron microscopes. Instruments at the "low" end of their price ranges are adequate for our uses, give excellent resolution (equal to or very near high-end models) and considerable flexibility, and are well suited to the research projects in this proposal, as well as likely future projects. The Philips (FEI) model Morgani 268 is a 100 kV microscope with a conventional motorized stage with a 6 degree tilt & high-resolution digital camera system. The microscope is fully digitized, controlled by an upgradable PC that is also used for digital image capture. This is a recently introduced model in the Philips line, and is the most modern of those considered. It offers exceptional value; is designed for the "cell biology, pathology, ultrastructural molecular biology" category of users; and is well suited to our needs. Unlike models that are in the middle or near the end of their product runs, this unit is at the beginning of its production period. Consequently, the microscope will be supported by the manufacturer with service and parts further into the future than the others, giving it a longer useful life span. Therefore, due to capability, versatility, needs appropriateness, upgradability, and overall value, we have specified the Philips Morgani 268 in this funding request.