MOLECULAR DYNAMICS STORM 860 IMAGING SYSTEMS

分子动力学 STORM 860 成像系统

• 批准号: 2516740
• 负责人: RICHARD A ASHMUN
• 金额: $ 11.01万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-25 至 1999-03-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2516740
• 关键词: biomedical equipment purchase; clinical biomedical equipment; digital imaging; fluorescence; imaging /visualization /scanning; molecular dynamics; phosphorescence

Funding is requested for two Molecular Dynamics Storm 860 Imaging Systems, to be established as shared instrument resources within St. Jude Children's Research Hospital. These instruments will replace two existing Molecular Dynamics PhosphorImager Systems which are heavily used by a large number of NIH grant-funded investigators. The specific advantages of the Storm instruments over existing PhosphorImagers or alternative technologies include: (a) the ability to rapidly detect and quantitate direct fluorescence for analysis of nucleic acids or proteins in gels; (b) the ability to detect enzyme-amplified chemifluorescence for fast analysis of nucleic acids or proteins on membranes; and (c) the complete retention of storage phosphor autoradiograph, capabilities of existing PhosphorImagers that are routinely used at this institution. The fluorescence-based techniques benefit from stable reaction products, higher sensitivity than comparable technologies, linearity of signal detection over a broader range than competing technologies, freedom from exposures to film, use of existing labelling protocols, and quantification of results and digital storage of images for future reference. These capabilities can immediately enhance many research projects that currently use either chemiluminescence labelling of proteins or nucleic acids on membranes, or routine staining and visualization of nucleic acids or proteins in gels by transillumination and/or photography. These instruments will be regularly employed by a major users group of 36 NIH grant-supported investigators from 11 departments, and will facilitate the efficient progress of multiple research programs.

为两个分子动力学风暴860成像申请资金 这些系统将在圣彼得堡建立为共享的仪器资源。 裘德儿童研究医院。这些仪器将取代两台 现有的分子动力学磷光成像系统 被美国国立卫生研究院拨款资助的大量调查人员使用。具体的 STORM仪器相对于现有磷光成像仪或 替代技术包括：(A)快速检测和 定量直接荧光用于核酸或蛋白质的分析 在凝胶中；(B)检测酶放大的化学荧光的能力 用于快速分析膜上的核酸或蛋白质；及(C) 完全保留存储磷光体放射自显像仪的能力 该机构经常使用的现有磷光成像仪。 基于荧光的技术受益于稳定的反应产物， 比同类技术更高的灵敏度，信号的线性度 与竞争技术相比，检测范围更广，不受 暴露在胶片中，使用现有的标签方案，以及 结果的量化和图像的数字存储以备将来 参考资料。这些能力可以立即加强许多研究 目前使用化学发光标记的项目 膜上的蛋白质或核酸，或常规染色和 透光显示凝胶中的核酸或蛋白质 和/或摄影。这些仪器将由一家 主要用户组，由36名NIH拨款支持的调查人员组成，来自11名 部门，并将促进多个部门的有效进展 研究项目。