Flow Cytometry and Cell Sorting Shared Resources Core

流式细胞术和细胞分选共享资源核心

• 批准号: 10582662
• 负责人: RICHARD A ASHMUN
• 金额: $ 29.55万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10582662
• 关键词: Acute Lymphocytic Leukemia; Acute Myelocytic Leukemia; Advanced Malignant Neoplasm; Advisory Committees; Area; Biological Assay; Biomedical Engineering; Blood; Cancer Center Support Grant; Cell Cycle; Cell Separation; Cells; Childhood Solid Neoplasm; Collaborations; Complex; Consultations; Cytometry; Data Collection; Dedications; Development; Doctor of Philosophy; Ensure; Equipment; Evaluation; Experimental Designs; Flow Cytometry; Fostering; Funding; Future; Goals; Human Resources; Image; Investments; Journals; Malignant Neoplasms; Methods; Nature; Oncology; Peer Review; Pharmacotherapy; Phenotype; Population; Preparation; Process; Protocols documentation; Publications; Reagent; Reporting; Research; Resource Sharing; Sampling; Services; Specialist; Supervision; Techniques; Technology; Testing; Time; Training; Training Programs; Validation; Work; Xenograft Model; anticancer research; cost; data complexity; experience; experimental study; instrument; instrumentation; investigator training; member; neoplastic cell; operation; osteosarcoma; programs; repository; response; skills; theories; tumor progression

PROJECT SUMMARY/ABSTRACT—Flow Cytometry and Cell Sorting Shared Resource The goal of the Flow Cytometry and Cell Sorting Shared Resource (FCCSSR) is to support SJCCC members by providing access to a wide variety of state-of-the-art flow cytometry equipment, services, expertise, and training. This includes provision of consultation regarding experimental design and interpretation of results, delivery of extensive training in the operation of high-end flow analyzers, and provision of 4 high-end analyzers for use by trained users. Dr. Richard Ashmun serves as the Director of the FCCSSR and is responsible for consultations with users, personnel supervision, training, instrument operation, and oversight of all aspects of the operation of this facility. Ashmun holds a PhD in biomedical engineering and has served as the highly successful Director of the FCCSSR since 1987. The FCCSSR staff includes three senior flow cytometry specialists and 5 other staff members. The staff operates 9 flow analyzers (4 provided for user operation) and 5 cell sorters. In the current reporting period the FCCSSR acquired 3 instruments: a BD Symphony analyzer, a BD Aria Fusion sorter, and an additional BD LSR II analyzer. The FCCSSR was used by 47 SJCCC members in FY17 and supported work from 4 of the 5 Programs (HMP, DBSTP, NBTP, and CBP). Among the SJCCC members using the facility, 77% (n=36) have cancer-related, peer-reviewed funding. This work resulted in 112 peer-reviewed publications, 34.8% from intraprogrammatic and 26.8% from interprogrammatic collaborations, with many in high-impact journals such as Nature, Cell, Lancet Oncology, and Blood. The contribution of the FCCSSR is exemplified by several key accomplishments. For example, the FCCSSR worked extensively with members of the HMP (Drs. Charles Mullighan, Tanja Gruber, Mary Relling, and William Evans) in studies of acute lymphoblastic leukemia and acute myeloid leukemia to isolate phenotypically characterized tumor cells for further study, track tumor progression in xenograft models, and isolate genetically modified populations using cell sorting. Additionally, the FCCSSR was an important part of work by the DBSTP, contributing to studies of cell cycle responses to drug treatment and the phenotypic characterization and isolation of osteosarcomas and other pediatric solid tumors. Future plans include testing of complex flow assays and evaluation of instrumentation for enhancement of current services, including possible expansion into additional areas such as image-based or mass cytometry. FCCSSR will continue working with SJCCC members to develop and validate new protocols, optimizing the use of new reagents, and expanding the repository of reagents available to users. Additionally, as the robustness and complexity of data emerging from the new instruments increases, the FCCSSR Director and Staff will dedicate more time for detailed consultations with users regarding flow techniques, will expand the in-house training program, and will carefully evaluate replacements for obsolete equipment through demonstrations, testing and discussions with end users and the FCCSSR Advisory Committee.

项目概要/摘要-流式细胞术和细胞分选共享资源 流式细胞术和细胞分选共享资源（FCCSSR）的目标是通过以下方式支持SJCCC成员 提供各种最先进的流式细胞仪设备、服务、专业知识和培训。 这包括提供有关实验设计和结果解释的咨询， 提供高端流量分析仪操作方面的广泛培训，并提供4台高端分析仪，供 训练有素的用户。Richard Ashmun博士担任FCCSSR主任，负责咨询 与用户，人员监督，培训，仪器操作，并监督操作的各个方面 这个设施。Ashmun拥有生物医学工程博士学位，曾担任非常成功的 自1987年以来，FCCSSR。FCCSSR的工作人员包括三名高级流式细胞仪专家和五名其他工作人员 成员工作人员操作9台流量分析仪（4台供用户操作）和5台细胞分选仪。在当前 报告期内，FCCSSR购买了3台仪器：BD Symphony分析仪、BD Aria Fusion分类仪和 一台额外的BD LSR II分析仪。FCCSSR在2017财年被47个SJCCC成员使用， 5个程序中的4个（HMP、DBSTP、NBTP和CBP）。在使用该设施的SJCCC成员中，77% （n = 36）有癌症相关的，同行评审的资金。这项工作产生了112篇同行评议的出版物，占34.8%。 26.8%来自项目内合作，其中许多来自高影响力期刊 如《自然》、《细胞》、《柳叶刀肿瘤学》和《血液》。FCCSSR的贡献体现在以下几个方面： 关键成就。例如，FCCSSR与HMP成员（Charles博士 Mullighan，Tanja Gruber，玛丽雷林和威廉埃文斯）在急性淋巴细胞白血病和 急性髓性白血病，以分离具有表型特征的肿瘤细胞用于进一步研究，跟踪肿瘤 在异种移植模型中的进展，并使用细胞分选分离遗传修饰的群体。另夕h FCCSSR是DBSTP工作的重要组成部分，有助于研究细胞周期对药物的反应 骨肉瘤和其他儿科实体瘤的治疗和表型表征和分离。 未来的计划包括测试复杂的流动分析和评估仪器，以提高电流 服务，包括可能扩展到其他领域，如基于图像或质谱仪。FCCSSR 将继续与SJCCC成员合作，开发和验证新的协议，优化新的 试剂，并扩大可供用户使用的试剂库。此外，由于鲁棒性和 新仪器产生的数据的复杂性增加，FCCSSR主任和工作人员将致力于 有更多的时间与用户就流动技术进行详细协商，将扩大内部培训 计划，并将通过演示，测试和 与最终用户和FCCSSR咨询委员会进行讨论。