MULTIUSER CONFOCAL MICROSCOPE IMAGING SYSTEM

此共享仪器授权应用程序请求购买资金 Biorad MRC-1024紫外可见光/紫外双激光共焦成像系统 位于新建研究中心的共用成像设备 设施。该系统将为该应用程序的九个主要用户提供服务 来自肯塔基大学多个系的调查人员 校园里。Biorad系统的配置满足了多- 用户组对荧光标记进行高分辨率成像 活的和固定的细胞和组织中的结构。要执行这些操作 功能Biorad系统将配置1)氪/Ar 用于激发三个不同波长(448 nm、568 nm和 647 nm)以允许三个荧光探针成像，2)紫外光/ 激发波长351 nm、363 nm(UV)的可见光Ar激光器 范围)和488 nm(可见范围)，以允许UV激发的探测器成像， 3)尼康Dapapot300倒置显微镜一台，配备相位镜和 荧光/紫外线配件(由肯塔基大学提供)，以及 4)康柏奔腾电脑，配备1.2 GB硬盘、32MB内存、CD-ROM、 1.44MB软盘和21英寸高分辨率显示器，用于控制和数据 收购。类似配置的可访问以太网的计算机 配备Codonic NP1600-BR照相打印机的工作站将 由肯塔基大学提供。该工作站将允许 对捕获的图像和采集的数据的处理与 共焦显微镜站，是满足这一需求所必需的 多用户设施。两个共焦系统现在在华盛顿大学 肯塔基州，主要用户组不可用，并且 另一个近六岁的人，其成像能力相当有限， 分析能力。将这一最先进的系统添加到 成像核心设施将为以下工作提供必要资源 在该专业的研究项目中描述的高级图像分析 用户以及大学科学界的其他人。

This Shared Instrumentation Grant application requests funds to purchase a Biorad MRC-1024UV visible light/UV dual laser confocal imaging system for a shared-use Imaging Facility located in a newly constructed research facility. This system will serve the nine Major Users of this application and investigators from multiple departments on the University of Kentucky campus. The Biorad system was configured to meet the needs of a multi- user group to carry out high resolution imaging of fluorescent labeled structures in both living and fixed cells and tissues. To perform these functions the BIORAD system will be configured with 1) a krypton/argon laser for excitation at three distinct wavelengths (448nm 568nm, and 647nm) to allow imaging of three fluorescent probes, 2) an ultraviolet/ visible argon laser with excitation wavelengths at 351nm, 363nm (UV range), and 488nm (visible range) to allow imaging of UV-excited probes, 3) a Nikon diaphot 300 inverted microscope equipped with phase optics and fluorescent/UV accessories (supplied by the University of Kentucky), and 4) a Compaq pentium computer with a 1.2GB hard drive, 32MB RAM, CD-ROM, 1.44MB floppy and a 21-inch high resolution monitor for control and data acquisition. A similarly configured ethernet accessible computer workstation equipped with Codonic NP1600-BR photographic printer will be supplied by the University of Kentucky. The workstation will allow manipulation of captured images and acquired data separate from the confocal microscope station and is essential to meet the needs of this multi-user facility. Two confocal systems are now at the University of Kentucky, one that is not available for use by the Major User group and another nearly six years of age that is quite limited in its imaging and analysis capabilities. The addition of this state-of-the-art system to the Imaging Core Facility will provide and essential resource for the advanced image analysis described in the research projects of the Major Users as well as others in the University's scientific community.