Cross-talk between the colon epithelium, colon afferents and sympathetic neurons regulate pain in the normal and inflamed colon

结肠上皮、结肠传入神经和交感神经元之间的串扰调节正常和发炎结肠的疼痛

基本信息

项目摘要

SUMMARY Visceral pain is notoriously difficult to treat, often persisting long after the precipitating injury/disease is no longer evident. In this application we will explore a novel, multicellular peripheral circuit that we hypothesize explains many of the intractable features of chronic, visceral pain. We now know that epithelial-neuronal communication is widespread, with numerous epithelial cell types releasing neuroactive substances (e.g., ATP, ACh, 5HT, glutamate). This is particularly apparent in the colon where we have found that channelrhodopsin (ChR2) -induced activation of colon epithelial cells produces high frequency bursting of colon extrinsic primary afferent neurons (ExPAN’s), phenocopying physiologic stimuli and inducing robust behavioral responses (visceromotor responses (VMR), a validated assay of hypersensitivity). Building on these findings, new surprising data indicate colon epithelium also receives functional input from sympathetic neurons; activation of sympathetic projections to the colon induces large, phase-locked calcium signals in the epithelium. Closing the loop, we found that activation of ExPAN’s via colorectal distension (CRD) induces calcium signals in the post-ganglionic sympathetic neurons projecting to the colon, and that ChR2- induced activation of ExPAN’s induces cFos expression in these same neurons. That this multicellular circuit plays a role in visceral pain is supported further by preliminary data that shows that inflammation (acute and/or chronic) is correlated with increased signaling in all portions of this circuit. Thus, the goal of the proposed experiments is to test the hypothesis that persistent visceral hypersensitivity is due, at least in part, to amplification in an epithelial-ExPAN-sympathetic circuit such that it is possible to treat pain by breaking any limb of this feed-forward circuit (Fig.1). This hypothesis will be tested in 3 aims: Aim 1: Determine if persistent hypersensitivity induced in a model of IBD (DSS (dextran sulfate sodium)) is due to increased epithelial signaling and/or ExPAN excitability, Aim 2: Determine if DSS-induced inflammation increases the ability of ExPANs to activate sympathetic neurons in prevertebral sympathetic ganglion (PrSG) directly (via synapses in PrSG) or indirectly (via a spinal cord circuit) and, Aim 3 Determine the ability of sympathetic neurons to drive activity in epithelial cells in naïve mice and in the DSS model of IBD.
总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Kathryn Marie Albers其他文献

Kathryn Marie Albers的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Kathryn Marie Albers', 18)}}的其他基金

Epidermal Nonpeptidergic Nerves Modulate Cutaneous Immunity
表皮非肽能神经调节皮肤免疫
  • 批准号:
    10216987
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Epidermal Nonpeptidergic Nerves Modulate Cutaneous Immunity
表皮非肽能神经调节皮肤免疫
  • 批准号:
    10652420
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Cross-talk between the colon epithelium, colon afferents and sympathetic neurons regulate pain in the normal and inflamed colon
结肠上皮、结肠传入神经和交感神经元之间的串扰调节正常和发炎结肠的疼痛
  • 批准号:
    10159250
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Molecular and Functional Analysis of Hirschsprung Defects in Humans and Mouse
人类和小鼠先天性巨结肠缺陷的分子和功能分析
  • 批准号:
    10597975
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Cross-talk between the colon epithelium, colon afferents and sympathetic neurons regulate pain in the normal and inflamed colon
结肠上皮、结肠传入神经和交感神经元之间的串扰调节正常和发炎结肠的疼痛
  • 批准号:
    10617264
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Epidermal Nonpeptidergic Nerves Modulate Cutaneous Immunity
表皮非肽能神经调节皮肤免疫
  • 批准号:
    10440275
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Epidermal Nonpeptidergic Nerves Modulate Cutaneous Immunity
表皮非肽能神经调节皮肤免疫
  • 批准号:
    10065774
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Molecular and Functional Analysis of Hirschsprung Defects in Humans and Mouse
人类和小鼠先天性巨结肠缺陷的分子和功能分析
  • 批准号:
    10386806
  • 财政年份:
    2020
  • 资助金额:
    $ 45.89万
  • 项目类别:
Characterization of epithelial-neural communication
上皮神经通讯的表征
  • 批准号:
    9240592
  • 财政年份:
    2016
  • 资助金额:
    $ 45.89万
  • 项目类别:
Characterization of epithelial-neural communication
上皮神经通讯的表征
  • 批准号:
    9898306
  • 财政年份:
    2016
  • 资助金额:
    $ 45.89万
  • 项目类别:

相似海外基金

How Spinal Afferent Neurons Control Appetite and Thirst
脊髓传入神经元如何控制食欲和口渴
  • 批准号:
    DP220100070
  • 财政年份:
    2023
  • 资助金额:
    $ 45.89万
  • 项目类别:
    Discovery Projects
The mechanisms of the signal transduction from brown adipocytes to afferent neurons and its significance.
棕色脂肪细胞向传入神经元的信号转导机制及其意义。
  • 批准号:
    23K05594
  • 财政年份:
    2023
  • 资助金额:
    $ 45.89万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
GPR35 on Vagal Afferent Neurons as a Peripheral Drug Target for Treating Diet-Induced Obesity
迷走神经传入神经元上的 GPR35 作为治疗饮食引起的肥胖的外周药物靶点
  • 批准号:
    10315571
  • 财政年份:
    2021
  • 资助金额:
    $ 45.89万
  • 项目类别:
Neurobiology of Intrinsic Primary Afferent Neurons
内在初级传入神经元的神经生物学
  • 批准号:
    10477437
  • 财政年份:
    2021
  • 资助金额:
    $ 45.89万
  • 项目类别:
Neurobiology of Intrinsic Primary Afferent Neurons
内在初级传入神经元的神经生物学
  • 批准号:
    10680037
  • 财政年份:
    2021
  • 资助金额:
    $ 45.89万
  • 项目类别:
Neurobiology of Intrinsic Primary Afferent Neurons
内在初级传入神经元的神经生物学
  • 批准号:
    10654779
  • 财政年份:
    2021
  • 资助金额:
    $ 45.89万
  • 项目类别:
Neurobiology of Intrinsic Primary Afferent Neurons
内在初级传入神经元的神经生物学
  • 批准号:
    10275133
  • 财政年份:
    2021
  • 资助金额:
    $ 45.89万
  • 项目类别:
GPR35 on Vagal Afferent Neurons as a Peripheral Drug Target for Treating Diet-Induced Obesity
迷走神经传入神经元上的 GPR35 作为治疗饮食引起的肥胖的外周药物靶点
  • 批准号:
    10470747
  • 财政年份:
    2021
  • 资助金额:
    $ 45.89万
  • 项目类别:
Roles of mechanosensory ion channels in myenteric intrinsic primary afferent neurons
机械感觉离子通道在肌间固有初级传入神经元中的作用
  • 批准号:
    RGPIN-2014-05517
  • 财政年份:
    2018
  • 资助金额:
    $ 45.89万
  • 项目类别:
    Discovery Grants Program - Individual
Roles of mechanosensory ion channels in myenteric intrinsic primary afferent neurons
机械感觉离子通道在肌间固有初级传入神经元中的作用
  • 批准号:
    RGPIN-2014-05517
  • 财政年份:
    2017
  • 资助金额:
    $ 45.89万
  • 项目类别:
    Discovery Grants Program - Individual
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了