CORE--ANALYTICAL INSTRUMENTATION

核心——分析仪器

• 批准号: 6109952
• 负责人: Antonio Scarpa
• 金额: --
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-15 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6109952
• 关键词: biological signal transduction; biomedical equipment; biomedical equipment purchase; hormone receptor; vasoactive agent

The Analytical Instrumentation Core will provide all the projects and investigators of the Program Project with a variety of state-of-the-art instrumentation aimed at obtaining measurements in various cells of cell metabolism, ion fluxes, compartmentation of ionic gradients and metabolites, intracellular messengers, etc. as described in the individual sections of the proposal. The instrumentation available through Core C is comprised of several spectrophotometers, fluorometers, HPLC systems, electrometers, amperometric detectors, etc. Part of the instrumentation has been produced commercially and part has been built inhouse and is continually upgraded to fulfill specific research needs. the staff of the core, who has proven expertise in the measurements proposed and in developing state-of-the-art technology, will provide assistance and counseling in the measurements described in the individual projects of this application. The measurements that Core C will carry out include: a) measurements of free intracellular H+ and Ca2+ in cell suspensions, cell monolayers and individual cells; b) kinetic measurement of ion transport across cell and cell membranes; c) microfluorimetric assays of P450 and other enzymatic reaction in small volumes or few cells; d) imaging of cell fluorescence (single cells or several cells) for H+or Ca2+ free intracellular distribution, fluorescent antibody distribution, etc.; e) ion selective electrodes, atomic absorbance, cell permeabilization, etc.; and f) measurements of cell metabolism; ATP/ADP ratios; HPLC of phosphomeabolites, phosphoinositides, etc.

分析仪器核心将提供所有项目， 该计划项目的研究人员与各种国家的最先进的 旨在获得细胞的各种细胞中的测量的仪器 代谢，离子通量，离子梯度的区室化， 代谢物、细胞内信使等，如在 提案的各个部分。 可用的仪器 通过核心C，由几个温度计，荧光计， HPLC系统、静电计、安培检测器等。 仪器已经商业化生产，部分已经建成 并不断升级，以满足特定的研究需求。 核心工作人员，他们在测量方面具有公认的专业知识， 提出并开发最先进的技术，将提供 援助和咨询中所述的测量 本申请的个别项目。 核心C的测量结果 将进行的包括：a）测量细胞内游离H+， 细胞悬浮液、细胞单层和单个细胞中的Ca 2 +; B） 跨细胞和细胞膜离子转运的动力学测量; c） P450和其他酶促反应的微量荧光测定 d）细胞荧光成像（单细胞或多细胞）; 几个细胞）用于无H+或Ca 2+的细胞内分布， 荧光抗体分布等; e）离子选择性电极， 原子吸收、细胞透化等;和f）测量 细胞代谢; ATP/ADP比率;磷酸化蛋氨酸的HPLC， 磷酸肌醇等。