CORE--ANALYTICAL INSTRUMENTATION

分析仪器核心将提供所有项目和 拥有各种最先进技术的项目调查人员 旨在获得各种单元格中的测量结果的仪器 新陈代谢、离子通量、离子梯度划分和 代谢物、细胞内信使等，如 提案的各个部分。可用的仪器 通芯C由多个分光光度计、荧光计、 高效液相系统、静电计、安培检测器等。 仪器已经商业化生产，部分已经建成。 并不断升级以满足特定的研究需求。 核心的工作人员，他们在测量方面拥有公认的专业知识 提出并开发最先进的技术，将提供 中描述的测量方面的帮助和咨询 此应用程序的单个项目。核心C的测量 将进行的工作包括：a)测量细胞内游离H和 细胞悬液、细胞单层和单个细胞中的Ca2；b) 跨细胞和细胞膜离子传输的动力学测量；c) 微量荧光分光光度法测P450和其他酶反应 体积或极少细胞；d)细胞荧光成像(单细胞或 几个细胞)用于无氢或无钙的细胞内分布， 荧光抗体分布等；e)离子选择电极， 原子吸光度、细胞通透性等；以及f)测量 细胞代谢；三磷酸腺苷/二磷酸腺苷比率；磷脂代谢产物的高效液相色谱， 肌醇磷脂等。

The Analytical Instrumentation Core will provide all the projects and investigators of the Program Project with a variety of state-of-the-art instrumentation aimed at obtaining measurements in various cells of cell metabolism, ion fluxes, compartmentation of ionic gradients and metabolites, intracellular messengers, etc. as described in the individual sections of the proposal. The instrumentation available through Core C is comprised of several spectrophotometers, fluorometers, HPLC systems, electrometers, amperometric detectors, etc. Part of the instrumentation has been produced commercially and part has been built inhouse and is continually upgraded to fulfill specific research needs. the staff of the core, who has proven expertise in the measurements proposed and in developing state-of-the-art technology, will provide assistance and counseling in the measurements described in the individual projects of this application. The measurements that Core C will carry out include: a) measurements of free intracellular H+ and Ca2+ in cell suspensions, cell monolayers and individual cells; b) kinetic measurement of ion transport across cell and cell membranes; c) microfluorimetric assays of P450 and other enzymatic reaction in small volumes or few cells; d) imaging of cell fluorescence (single cells or several cells) for H+or Ca2+ free intracellular distribution, fluorescent antibody distribution, etc.; e) ion selective electrodes, atomic absorbance, cell permeabilization, etc.; and f) measurements of cell metabolism; ATP/ADP ratios; HPLC of phosphomeabolites, phosphoinositides, etc.