ETHANOLS ACTIONS IN GAMMA PKC NULL MUTANTS
乙醇在 GAMMA PKC 无效突变体中的作用
基本信息
- 批准号:2841998
- 负责人:
- 金额:$ 25.56万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1999
- 资助国家:美国
- 起止时间:1999-03-01 至 2002-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Protein phosphorylation is a basic regulator of cellular processes. One
gene family, the calcium/lipid activated protein kinase C (PKC) encodes
at least 11 isotypes. Among these isotypes the y-PKC isotype is the only
PKC solely expressed in neurons of the brain and spinal cord. Until the
creation of null mutant mice ("knock-outs") that carry a disrupted y-PKC
gene, it has been difficult to analyze the role of specific isotypes in
behavioral, biochemical, and physiological processes.
We have previously demonstrated that y-PKC null mutants are less
sensitive to the hypothermia-inducing and sedative-hypnotic effects of
alcohol compared to their wild-type littermates. We provide data here
showing that y-PKC mutants do not develop tolerance to some of alcohol's
effects. Therefore, it appears that the y-isotype of PKC may play a role
in the mediation of ethanol's action.
The goal of this proposal is to investigate the role of y-PKC in
determining behavioral responses to acute and chronic ethanol treatment.
In specific aim #1, the y-PKC null mutants will be compared to mice that
are either wild-type or heterozygous for the mutations using several
measures of initial sensitivity and tolerance. In specific aim #2,
transgenic mouse lines over-expressing y-PKC will be created and
characterized for initial sensitivity and tolerance to ethanol. A
genetic rescue of the y-PKC null mutants will also be performed by a
genetic cross between transgenics and null mutants.
The results of these studies will establish whether y-PKC plays a role
in the pharmacological actions of ethanol that underlie the
determination of ethanol sensitivity and tolerance.
蛋白质磷酸化是细胞过程的基本调节因子。一个
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JEANNE M WEHNER其他文献
JEANNE M WEHNER的其他文献
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{{ truncateString('JEANNE M WEHNER', 18)}}的其他基金
Role of Nicotinic Receptors in Effects of Alcohol
烟碱受体在酒精作用中的作用
- 批准号:
6881433 - 财政年份:2002
- 资助金额:
$ 25.56万 - 项目类别:
Role of Nicotinic Receptors in Effects of Alcohol
烟碱受体在酒精作用中的作用
- 批准号:
7028986 - 财政年份:2002
- 资助金额:
$ 25.56万 - 项目类别:
Role of Nicotinic Receptors in Effects of Alcohol
烟碱受体在酒精作用中的作用
- 批准号:
6477753 - 财政年份:2002
- 资助金额:
$ 25.56万 - 项目类别:
Role of Nicotinic Receptors in Effects of Alcohol
烟碱受体在酒精作用中的作用
- 批准号:
6727463 - 财政年份:2002
- 资助金额:
$ 25.56万 - 项目类别:
Role of Nicotinic Receptors in Effects of Alcohol
烟碱受体在酒精作用中的作用
- 批准号:
6625624 - 财政年份:2002
- 资助金额:
$ 25.56万 - 项目类别:
BEHAVIORAL STUDIES OF NICOTINIC RECEPTOR NULL MUTANTS
烟碱受体无效突变体的行为研究
- 批准号:
6201624 - 财政年份:1999
- 资助金额:
$ 25.56万 - 项目类别:
BEHAVIORAL STUDIES OF NICOTINIC RECEPTOR NULL MUTANTS
烟碱受体无效突变体的行为研究
- 批准号:
6216560 - 财政年份:1999
- 资助金额:
$ 25.56万 - 项目类别:
ETHANOLS ACTIONS IN GAMMA PKC NULL MUTANTS
乙醇在 GAMMA PKC 无效突变体中的作用
- 批准号:
6163747 - 财政年份:1999
- 资助金额:
$ 25.56万 - 项目类别:
ETHANOLS ACTIONS IN GAMMA PKC NULL MUTANTS
乙醇在 GAMMA PKC 无效突变体中的作用
- 批准号:
6362171 - 财政年份:1999
- 资助金额:
$ 25.56万 - 项目类别: