DIAGNOSTIC ADJUNCTS TO CYTOPATHOLOGICAL DIAGNOSIS

免疫细胞化学已被证明是一种有价值的 细胞学诊断的辅助诊断。细胞病理学 科室参与了几个临床相关的研究项目， 其中许多采用细针抽吸(FNA)和 免疫细胞化学提供辅助诊断信息 关于各种肿瘤抗原的表达，人类白细胞抗原或 其他记号笔。此类研究的部分清单包括：(1) 肿瘤细胞表达CD22、CD19百分率的测定 FNA标本作为使用单抗进行的临床试验的一部分 难治性淋巴瘤的治疗；(2)细胞学诊断和 眼淋巴瘤的免疫表型；(3)特征和 一种新型鼠源性抗癌单抗的应用 黑色素瘤、MART-1和随后的MART-1定量 恶性黑色素瘤患者的FNAS治疗 抗MART-1抗体；(4)形态和免疫细胞化学 对骨髓和外周血样本进行评估 目的检测IV期乳腺癌患者的恶性细胞。 (5)近期的细胞学和免疫细胞化学分析 用于恶性肿瘤鉴别诊断的描述性抗体 腺癌间皮瘤；(6)CD3定量 恶性黑色素瘤FNA标本中阳性淋巴细胞的检测 免疫治疗前后的患者；(7)利用 骨髓和采集物标本中的细胞角蛋白抗体 微小残留病与循环肿瘤细胞诊断 乳腺癌患者。7)免疫细胞化学评估 中国人支气管肺泡灌洗液标本中的淋巴组织 局部暴露于内毒素后的患者。“

"Immunocytochemistry has proved to be a valuable diagnostic adjunct to cytopathologic diagnosis. The Cytopathology Section is involved in several clinically-related research projects, many of which utilize fine needle aspiration (FNA) and immunocytochemistry to provide ancillary diagnostic information regarding expression of various tumor antigens, HLA antigens or other markers. A partial listing of such studies includes: (1) Evaluation of percent of tumor cells expressing CD22 and CD19 in FNA specimens as part of a clinical trial using monoclonal antibody therapy for refractory lymphomas;(2) Cytologic diagnosis and immunophenotyping of ocular lymphomas; (3) characterization and application of a novel murine monoclonal antibody for malignant melanoma, MART-1 with subsequent quantitation of MART-1 in FNAs from malignant melanoma patients for treatment with anti-MART-1 antibody; (4) morphologic and immunocytochemical evaluation of bone marrow and peripheral blood samples for the detection of malignant cells in patients with stage IV breast cancer; (5) cytologic and immunocytochemical analysis of recently describeld antibodies for the differentialtion of malignant mesothelioma from adenocarcinoma;(6) Quantitation of CD3 positive lymphocytes in FNA specimens in malignant melanoma patients before and after immunotherapy;(7) utilization of cytokeratin antibodies in bone marrow and apheresis specimens for minimal residual disease and circulating tumor cell diagnoses in breast carcinoma patients.7) immunocytochemical evaluation of lymphoid populations in bronchoalveolar lavage specimens from patients subsequent to local exposure to endotoxin. ."