DIAGNOSTIC ADJUNCTS TO CYTOPATHOLOGICAL DIAGNOSIS

细胞病理学诊断的辅助诊断

• 批准号: 6163416
• 负责人: A ABATI
• 金额: --
• 依托单位: DIVISION OF CLINICAL SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6163416
• 关键词: adolescence (12-20); biomarker; breast neoplasms; child (0-11); clinical research; cytodiagnosis; fine needle aspiration; human subject; human tissue; immunocytochemistry; lymphoma; melanoma; neoplasm /cancer diagnosis; tumor antigens

"Immunocytochemistry has proved to be a valuable diagnostic adjunct to cytopathologic diagnosis. The Cytopathology Section is involved in several clinically-related research projects, many of which utilize fine needle aspiration (FNA) and immunocytochemistry to provide ancillary diagnostic information regarding expression of various tumor antigens, HLA antigens or other markers. A partial listing of such studies includes: (1) Evaluation of percent of tumor cells expressing CD22 and CD19 in FNA specimens as part of a clinical trial using monoclonal antibody therapy for refractory lymphomas;(2) Cytologic diagnosis and immunophenotyping of ocular lymphomas; (3) characterization and application of a novel murine monoclonal antibody for malignant melanoma, MART-1 with subsequent quantitation of MART-1 in FNAs from malignant melanoma patients for treatment with anti-MART-1 antibody; (4) morphologic and immunocytochemical evaluation of bone marrow and peripheral blood samples for the detection of malignant cells in patients with stage IV breast cancer; (5) cytologic and immunocytochemical analysis of recently describeld antibodies for the differentialtion of malignant mesothelioma from adenocarcinoma;(6) Quantitation of CD3 positive lymphocytes in FNA specimens in malignant melanoma patients before and after immunotherapy;(7) utilization of cytokeratin antibodies in bone marrow and apheresis specimens for minimal residual disease and circulating tumor cell diagnoses in breast carcinoma patients.7) immunocytochemical evaluation of lymphoid populations in bronchoalveolar lavage specimens from patients subsequent to local exposure to endotoxin. ."

“免疫细胞化学已被证明是一种有价值的 细胞病理学诊断的辅助诊断。细胞病理学 部门参与了多个临床相关的研究项目， 其中许多利用细针抽吸 (FNA) 和 免疫细胞化学提供辅助诊断信息 关于各种肿瘤抗原、HLA抗原或 其他标记。此类研究的部分清单包括：(1) 评估表达 CD22 和 CD19 的肿瘤细胞百分比 FNA 标本作为使用单克隆抗体的临床试验的一部分 难治性淋巴瘤的治疗；（2）细胞学诊断和 眼淋巴瘤的免疫表型分析； (3) 表征和 新型鼠单克隆抗体在恶性肿瘤中的应用 黑色素瘤，MART-1，随后对 MART-1 进行定量 来自恶性黑色素瘤患者的 FNA 用于治疗 抗MART-1抗体； (4)形态学和免疫细胞化学 评估骨髓和外周血样本 IV期乳腺癌患者恶性细胞的检测； (5)最近的细胞学和免疫细胞化学分析 描述了用于鉴别恶性细胞的抗体 腺癌间皮瘤；(6) CD3 定量 恶性黑色素瘤 FNA 标本中淋巴细胞阳性 免疫治疗前后的患者；(7) 骨髓和血浆分离标本中的细胞角蛋白抗体 微小残留病和循环肿瘤细胞诊断 乳腺癌患者。7) 免疫细胞化学评估 支气管肺泡灌洗标本中的淋巴细胞群 局部接触内毒素后的患者。 ”。