CORE--QUANTITATIVE MORPHOLOGY

核心--定量形态学

• 批准号: 6232848
• 负责人: JOSEPH E LEDOUX
• 金额: $ 29.64万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6232848
• 关键词: amygdala; animal data; bioimaging /biomedical imaging; biomedical facility; brain mapping; computer assisted diagnosis; confocal scanning microscopy; electron microscopy; electrophysiology; glucocorticoids; glutamate receptor; hippocampus; hormone regulation /control mechanism; human data; neurochemistry; prefrontal lobe /cortex; stress

DESCRIPTION (Adapted from applicant's abstract): The Quantitative Morphology Core (QMC), directed by Dr. Patrick Hof, provides expertise with quantitative neuroanatomical methods and image analysis, as well as help in study design to the Center's investigators. An important feature of the QMC is its capability to cross levels of morphological resolution, from macroscopic features to subcellular characteristics, to provide a comprehensive understanding of neurochemical morphology, not only in the context of functional circuits, but also in relation to the behavioral and electrophysiological paradigms employed by the Center's investigators. The QMC is equipped with two computer-assisted photomicroscopes, one confocal laser scanning microscope and has full access to an electron microscope facility. Quantitative morphology is performed principally using NeuroZoom, a software application designed for full-scale mapping, cell reconstruction, image analysis and stereology. In view of the very high level of interaction in terms of quantitative anatomic analyses among the different members of the Center, a key role of the QMC is to generate a uniform approach to quantitative neuroanatomy in order to ensure a high level of consistency in analysis, statistics, and interpretation of the morphologic data. Specifically, the QMC will participate in collaborative studies of glucocorticoid and glutamate receptor localization in projection neurons in the medial prefrontal cortex and amygdala as well as in intra-amygdala circuits (with Projects 1 and 3), stereologic analysis of neuron numbers and structure volumes of select subfields in the hippocampus (with Project 4), and quantitative cellular reconstructions of physiologically characterized and dye-filled neurons to assess the degree of stress-induced dendritic atrophy in all three brain regions (with Projects 1 and 3). In addition, the QMC will continue to develop new methods to optimize sampling on confocal images and to permit stereology on electron microscopy materials. It will collaborate with Projects 2 and 4 to provide neuroanatomical expertise to interpret MRI scans from experimental animals and human subjects, particularly with respect to delineation of regional boundaries in the hippocampal formation, and will assist with statistical analyses of regional volumes estimates calculated from the MRI.

描述（改编自申请人摘要）：定量形态学核心（QMC）由Patrick Hof博士指导，提供定量神经解剖学方法和图像分析方面的专业知识，并为中心的研究人员提供研究设计方面的帮助。QMC的一个重要特征是它能够跨越形态学分辨率的水平，从宏观特征到亚细胞特征，不仅在功能电路的背景下，而且在与中心研究人员使用的行为和电生理范式相关的情况下，提供对神经化学形态的全面理解。QMC配备了两台计算机辅助的显微显微镜，一台共聚焦激光扫描显微镜，并可以完全使用电子显微镜设备。定量形态学主要使用NeuroZoom进行，这是一种设计用于全尺寸绘图、细胞重建、图像分析和立体学的软件应用程序。鉴于中心不同成员之间在定量解剖分析方面的高度互动，质量管理委员会的一个关键作用是产生一种统一的定量神经解剖学方法，以确保在分析、统计和解释形态学数据方面的高度一致性。具体而言，QMC将参与关于内侧前额叶皮层和杏仁核投射神经元中糖皮质激素和谷氨酸受体定位以及杏仁核内回路的合作研究（项目1和项目3），海马部分子区神经元数量和结构体积的体视学分析（项目4）。定量重建生理特征和染色神经元，以评估所有三个脑区应力诱导的树突萎缩程度（项目1和3）。此外，QMC将继续开发新的方法来优化共聚焦图像的采样，并允许电子显微镜材料的立体学。它将与项目2和项目4合作，提供神经解剖学专业知识，以解释来自实验动物和人类受试者的MRI扫描，特别是关于海马体形成区域边界的划定，并将协助对MRI计算的区域体积估计进行统计分析。