CORE--NEUROANATOMY AND BEHAVIOR

神经解剖学核心将继续为组成该计划的六个项目中的每个项目提供基本服务。核心将提供四部分服务：1)标准组织学染色，包括Nissl、Weigart、Timms、Bodian、Fink-Heimer；2)神经组织冷冻切片或石蜡切片抗原免疫细胞化学定位设备和试剂；3)原位杂交定位mRNA物种的设施和设备；4)用于电镜超微结构研究的设备和试剂，包括免疫电镜和定量分析。前三种成分已经在多德博士的实验室中提供；EM研究在贝利医生操作的第二个设施中进行。Drs。Dodd和Bailey在所采用的方法方面拥有丰富的相关经验。他们还将就试剂和设备的购买和使用作出决定，并在必要时经常开会，以评估核心所提供的服务。详细说明了各个项目对核心服务的预期使用情况。行为核心将由霍金斯博士领导，并将继续提供各种行为测试，以探索转基因小鼠和不同品系野生型小鼠的学习能力，以进行比较。这些设施对六个项目中的每一个都作出了重大贡献。该中心目前拥有用于小鼠海马体依赖（外显）学习测试的设备（例如Morris水迷宫、桡臂迷宫、物体识别）。此外，核心区域有内隐记忆任务（例如，莫里斯水迷宫的提示版本，味觉厌恶，气味增强惊吓）。最后，核心计划开发海马体依赖和独立学习的额外测试（例如，延迟不匹配样本，视觉配对比较和经典条件反射的高阶方面）。并非所有项目都利用这一核心，但鉴于中心的总体目标，这是必不可少的。这些是在过去五年中持续提供基础设施支持的核心。核心的基本原理是，集中这些功能应该导致跨项目的技术一致性和操作规模经济。这两个核心组成部分都符合中心的目标

The Neuroanatomical Core will continue to provide essential services for each of the six projects comprising this program. There will be four components of the services provided by the core: 1) standard histological stains, including Nissl, Weigart, Timms, Bodian, and Fink-Heimer; 2) equipment and reagents for the immunocytochemical localization of antigens in frozen or paraffin sections of neural tissue; 3) facilities and equipment for the localization of mRNA species by in situ hybridization; and 4) facilities and reagents for ultrastructural studies at the electron microscopic level, including immunoelectron microscopy and quantitative analyses. The first three of these components have been made available in the laboratory of Dr. Dodd; the EM studies are performed in a second facility operated by Dr. Bailey. Drs. Dodd and Bailey have extensive relevant experience in the methodologies to be employed. They will also make decisions on the purchase and use of reagents and equipment, and they will meet as often as is necessary to evaluate the services provided by the core. The expected use of the core services by individual projects is detailed. The Behavioral Core will be headed by Dr. Hawkins and will continue to provide a variety of behavioral tests to explore learning capabilities in transgenic mice and different strains of wild-type mice for comparison. These facilities have contributed significantly to each of the six projects. The core currently has facilities for several tests of hippocampal-dependent (explicit) learning in mice (e.g., Morris water maze, radial arm maze, object recognition). In addition, the core has tasks of implicit memory (e.g., a cued version of the Morris water maze, taste aversion, odor potentiated startle). Finally, the core plans to develop additional tests of both hippocampal-dependent and-independent learning (e.g., delayed non-match to sample, visual paired comparison, and higher-order aspects of classical conditioning). Not all projects make use of this core, but it is essential given the overall aims of the Center. These are continuing cores that have provided infrastructure support during the past five years. The rationale for the core is that centralizing these functions should lead to consistency in technology across projects and economies of scale of operation. Both of these core components are justified by the aims of the Center