STRUCTURE OF MAJOR D1 CHAIN OF GIANT HEMOGLOBIN FROM LUMBRICUS TERRISTRIS

地龙巨血红蛋白 D1 主链的结构

• 批准号: 6118276
• 负责人: AUSTEN F RIGGS
• 金额: $ 0.09万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-10 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118276
• 关键词: biomedical resource; infection; parasitism; spectrometry; vaccines

The mass spectrometric analysis of the major chain d1, one of the four heme-binding chains in earthworm hemeglobin of Lumbricus terrestris, revealed a difference between the measured and the sequence derived masses indicated errors in the resported sequence. The purpose of this study is to clarify the possible sequence ambiguity by mass spectrometric peptide mapping experiments. After the digestion of chain d with a number of enzymes, proteolytic peptides in the region of 51-140 were identified and indicated a correct sequence assignment in this region. In addition, digestin withV8 protease revealed an sequence error in position 58 (E instead of Q). N-terminal Edman sequencing allowed the correction of misidentified amino acids 7 (G7S), 23 (Q23E) and 33 (K33R). The refined sequence (1-140) results in a molecular mass of resolution of these resolution of these discrepancies has been published Q, Zie, R.A. Donahue Jr., K. Schneider, U.A. Mirza, I. Haller, B.T. C hait & A.F . Riggs "Structure of chain d of the gigantic hemoglobin of the earthworm" BBA 1337, (1997) 241-247.

主链 d1（其中之一）的质谱分析 蚯蚓血红蛋白中的四个血红素结合链 terrestris，揭示了测量值和实际值之间的差异 序列衍生的质量表明报告的序列中存在错误。 本研究的目的是阐明可能的顺序 质谱肽图谱实验的模糊性。 后 用多种酶消化 d 链，蛋白水解 51-140 区域中的肽被鉴定并表明 该区域的正确序列分配。 此外，消化素 withV8 蛋白酶揭示了第 58 位的序列错误（E 改为 Q）。 N 端 Edman 测序允许校正 错误识别氨基酸 7 (G7S)、23 (Q23E) 和 33 (K33R)。 这 精炼序列 (1-140) 的分辨率为 这些差异的解决方案已发布 Q、Zie、 R.A. Donahue Jr.、K. Schneider，美国米尔扎 (Mirza)，I. 哈勒 (B.T. Haller)柴特& AF. 里格斯“巨大血红蛋白的 d 链结构 蚯蚓”BBA 1337, (1997) 241-247。