STRUCTURE OF MAJOR D1 CHAIN OF GIANT HEMOGLOBIN FROM LUMBRICUS TERRISTRIS

主链D_1的质谱分析 蚯蚓半球蛋白中的四条血红素结合链 Terrestris，揭示了被测量的和被测量的 序列衍生的肿块表明报告的序列中有错误。 这项研究的目的是澄清可能的顺序 通过质谱肽图谱实验获得多义性。之后 用一些酶消化d链，蛋白分解 鉴定了51-140区段的多肽，并表明 该区域的序列分配正确。此外，消化 V8蛋白酶显示58位(而不是E)的序列错误 Q)。N-末端埃德曼测序允许纠正 错误识别的氨基酸7(G7S)、23(Q23E)和33(K33R)。这个 精制序列(1-140)导致分子质量的分辨率为 这些差异的解决方案已经发表了，齐， 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 A.F.巨型血红蛋白d链的Riggs“结构 《蚯蚓》BBA 1337，(1997)241-247。

The mass spectrometric analysis of the major chain d1, one of the four heme-binding chains in earthworm hemeglobin of Lumbricus terrestris, revealed a difference between the measured and the sequence derived masses indicated errors in the resported sequence. The purpose of this study is to clarify the possible sequence ambiguity by mass spectrometric peptide mapping experiments. After the digestion of chain d with a number of enzymes, proteolytic peptides in the region of 51-140 were identified and indicated a correct sequence assignment in this region. In addition, digestin withV8 protease revealed an sequence error in position 58 (E instead of Q). N-terminal Edman sequencing allowed the correction of misidentified amino acids 7 (G7S), 23 (Q23E) and 33 (K33R). The refined sequence (1-140) results in a molecular mass of resolution of these resolution of these discrepancies has been published Q, Zie, R.A. Donahue Jr., K. Schneider, U.A. Mirza, I. Haller, B.T. C hait & A.F . Riggs "Structure of chain d of the gigantic hemoglobin of the earthworm" BBA 1337, (1997) 241-247.