STRUCTURE OF THE MAJOR D1, CHAIN OF GIANT HEMOGLOBIN FROM LUMBRICUS TERRISTRIS

主要 D1 的结构，来自地龙的巨血红蛋白链

• 批准号: 6279474
• 负责人: AUSTEN F RIGGS
• 金额: $ 0.08万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6279474
• 关键词: Invertebrata; biological products; biomedical resource; proteins; spectrometry

The mass spectrometric analysis of the major chain d1, one of the four heme-binding chains in earthworm hemeglobin of Lumbricus terrestris, revealed a difference between the measured and the sequence derived masses indicating errors in the reported sequence. The purpose of this study is to clarify the possible sequence ambiguity by mass spectrometric peptide mapping experiments. After the digestion of chain d with a number of enzymes, proteolytic peptides in the region of 51-140 were identified and indicated a correct sequence assignment in this region. In addition, digestion with V8 protease revealed an sequence error in position 58 (E instead of Q). N-terminal Edman sequencing allowed the correction of misidentified amino acids 7 (G7S), 23 (Q23E) and 33 (K33R). The refined sequence (1-140) results in a molecular mass of 15961 Da which is 28 Da below the measured mass of 15989Da. A paper describing the resolution of these discrepancies has been published Q. Xie, R. A. Donahue, Jr., K. Schneider, U.A. Mirza, I. Haller, B.T. Chait & A. F. Riggs "Structure of chain d of the gigantic hemoglobin of the earthworm" BBA 1337 (1997) 241-247.

主链D_1的质谱分析 蚯蚓半球蛋白中的四条血红素结合链 Terrestris，揭示了被测量的和被测量的 序列派生的团块指示报告序列中的错误。 这项研究的目的是澄清可能的顺序 通过质谱肽图谱实验获得多义性。之后 用一些酶消化d链，蛋白分解 鉴定了51-140区段的多肽，并表明 该区域的序列分配正确。此外，消化 用V8蛋白酶显示58位序列错误(而不是E Q)。N-末端埃德曼测序允许纠正 错误识别的氨基酸7(G7S)、23(Q23E)和33(K33R)。这个 精制序列(1-140)得到15961 Da的相对分子质量 比15989Da的测量质量低28Da。一篇描述这一现象的论文 这些差异的解决方案已发表在谢启超的文章中。 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 巨型血红蛋白d链的Riggs“结构 蚯蚓“BBA 1337(1997)241-247.