SEQUENCE ANALYSIS OF CHARGE DERIVATIZED PEPTIDES BY ELECTROSPRAY MASS SPEC

通过电喷雾质谱对电荷衍生化肽进行序列分析

• 批准号: 6258784
• 负责人: JACK T WATSON
• 金额: $ 0.19万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6258784
• 关键词: biological products; biomedical equipment development; biomedical resource; carbohydrates; technology /technique development

Methodology for the preparation and analysis of N-tris(2,4,6-trimethoxyphenyl)phosphonium-acetyl (TMPP-Ac) derivatives of peptides has been developed in this laboratory. Most often we analyze the TMPP-Ac derivatives of peptides by FAB with subsequent CAD or by MALDI with subsequent PSD to obtain sequence information. We are investigating the analysis of the TMPP-Ac derivatives by ESI with in-source fragmentation to obtain sequence information. Our preliminary experiments show that in-source fragmentation of the TMPP-Ac derivatized peptides is sensitive to different parameters such as cone voltage, capillary voltage, nebulizing gas flow rate, etc. The developing methodology is being extended to TMPP-Ac derivatization of peptide fragments from tryptic digests of proteins with subsequent analysis by LC-MS using electrospray.

用于制备和分析的方法学 N-tris(2，4，6-trimethoxyphenyl)phosphonium-acetyl(TMPP-Ac)衍生物 在这个实验室里已经开发出了一系列多肽。大多数情况下，我们 Fab与后续CAD联用分析多肽的TMPP-Ac衍生物 或者通过具有后续PSD的MALDI来获得序列信息。我们 正在研究TMPP-Ac衍生物的电喷雾化分析 源代码内分段以获取序列信息。我们的 初步实验表明，源代码内碎片化 TMPP-Ac衍生肽对不同的参数敏感 如锥体电压、毛细管电压、雾化气体流量等。 开发的方法学正在扩展到tmpp-ac衍生化。 从胰酶消化的蛋白质中分离出的多肽片段 电喷雾LC-MS分析。