AMINO TERMINAL PROCESSING OF YEAST PROTEINS

酵母蛋白的氨基末端加工

• 批准号: 6118496
• 负责人: YIE-HWA CHANG
• 金额: $ 0.11万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118496
• 关键词: biomedical resource; parasitism; proteins; spectrometry

Mutant Glutathione-S-Transferases (GST) were expressed and purified from wild-type yeast, map1 yeast, and map2 yeast. Since methionine aminopeptidases (MetAPs) are involved in N-terminal processing, we predict that GSTs expressed in yeast strains lacking either MetAP1 or MetAP2 should not have their N-terminal methionine completely removed. We hope using mass spectrometry to analyze the extent of met-removal of different GSTs to understand the roles of two distinct MetAPs. We are using a series of mutant glutathionine-S-transferases to evaluate the roles of each methionine aminopeptidases in eukaryotic cells.

突变型谷胱甘肽- s -转移酶（GST）表达