SBIR TOPIC 294 PHASE 1 - DEVELOPMENT OF GLYCOSYLATION - SPECIFIC RESEARCH

SBIR 主题 294 第 1 阶段 - 糖基化的发展 - 特定研究

• 批准号: 8353245
• 负责人: YIE-HWA CHANG
• 金额: $ 14.86万
• 依托单位: MEDIOMICS, LLC
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2012-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8353245
• 关键词: Antibodies; Antigens; Biological Assay; Cancer Biology; Carbohydrates; Chickens; Development; Early Diagnosis; Glycoproteins; Goals; Human; IgY; Image; Link; Malignant - descriptor; Malignant Neoplasms; Methods; Modification; Pattern; Peptides; Phase; Post-Translational Protein Processing; Protein Glycosylation; Proteins; Reagent; Research; Research Personnel; Site; Small Business Innovation Research Grant; Specificity; Staging; Technology; Testing; Time; cancer diagnosis; cancer therapy; cancer type; glycosylation; instrument; polyclonal antibody; tumor progression

Glycosylation changes are a universal feature of malignant transformation and tumor progression. Changes in expression levels of certain glycoproteins and altered glycosylation patterns have been detected in most of the major human cancer types and at different stages of tumor progression. The early detection of glycosylation changes is therefore crucial for cancer diagnosis and treatment. Currently, the methods for glycosylation analysis require expensive instruments and are time consuming. Thus, there is a need to develop new reagents and simpler methods to study carbohydrate modifications of proteins in cancer biology. Our long term goal is to develop new research reagents for basic cancer researchers in the form of antibodies against N- or O-linked carbohydrate antigens in proteins. In the Phase I proposal, we propose to generate over 32 chicken polyclonal (Task 1) and 2-3 monoclonal IgY (Task 2) ¿ against specific Oglycosylation sites. In addition, we will use our proprietary PINCER¿ platform technology to develop 5 costeffective, simple and fast assays (Task 3) to detect protein glycosylation using existing commercially available antibodies. We expect that these PINCER¿ reagents can be used to not only develop homogeneous assays for detecting protein glycosylation, but can also be used as unique imaging reagents.

糖基化变化是恶性转化和肿瘤进展的普遍特征。变化