SBIR TOPIC 294 PHASE 1 - DEVELOPMENT OF GLYCOSYLATION - SPECIFIC RESEARCH

糖基化改变是恶性转化和肿瘤进展的普遍特征。变化 在某些糖蛋白的表达水平和改变的糖基化模式中， 主要的人类癌症类型和肿瘤进展的不同阶段。的早期检测 因此，糖基化变化对于癌症诊断和治疗至关重要。目前，用于 糖基化分析需要昂贵的仪器且耗时。因此，有必要 开发新的试剂和更简单的方法来研究癌症中蛋白质的糖修饰 生物学我们的长期目标是为基础癌症研究人员开发新的研究试剂， 针对蛋白质中N-或O-连接的糖抗原的抗体。在第一阶段的建议中，我们建议 产生超过32个鸡多克隆抗体（任务1）和2 - 3个单克隆IgY（任务2）？ 网站.此外，我们将利用我们专有的PINCER平台技术开发5个成本效益， 使用现有的商业上可用于检测蛋白质糖基化的简单和快速的测定（任务3） 可用的抗体。我们希望这些PINCER试剂不仅可以用于开发 本发明提供了用于检测蛋白质糖基化的均相测定法，但也可用作独特的成像试剂。

Glycosylation changes are a universal feature of malignant transformation and tumor progression. Changes in expression levels of certain glycoproteins and altered glycosylation patterns have been detected in most of the major human cancer types and at different stages of tumor progression. The early detection of glycosylation changes is therefore crucial for cancer diagnosis and treatment. Currently, the methods for glycosylation analysis require expensive instruments and are time consuming. Thus, there is a need to develop new reagents and simpler methods to study carbohydrate modifications of proteins in cancer biology. Our long term goal is to develop new research reagents for basic cancer researchers in the form of antibodies against N- or O-linked carbohydrate antigens in proteins. In the Phase I proposal, we propose to generate over 32 chicken polyclonal (Task 1) and 2-3 monoclonal IgY (Task 2) ¿ against specific Oglycosylation sites. In addition, we will use our proprietary PINCER¿ platform technology to develop 5 costeffective, simple and fast assays (Task 3) to detect protein glycosylation using existing commercially available antibodies. We expect that these PINCER¿ reagents can be used to not only develop homogeneous assays for detecting protein glycosylation, but can also be used as unique imaging reagents.