INTERACTION BETWEEN GENE EXPRESSION & DNA DAMAGE DUE TO IONIZING RADIATION

基因表达之间的相互作用

• 批准号: 6118590
• 负责人: JOSEPH L ROTI ROTI
• 金额: $ 0.04万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118590
• 关键词: biomedical resource; biotechnology; human tissue; nucleic acids; spectrometry

A major goal of this research is to synthesize model systems of ionizing radiation damaged DNA and study the effect on transcription in vitro and in vivo. Our recent work has been specifically focused on the design and synthesis of photocleavable DNA building blocks for the production of "caged" DNA single and double strand breaks. Routine high resolution mass spectrometry is required to characterize intermediates in the synthesis of these building blocks

这项研究的一个主要目标是综合模型系统 电离辐射对DNA的损伤及其对转录的影响 在体外和体内。我们最近的工作特别集中在 可光解DNA构筑块的设计与合成 生产“笼子”中的DNA单链和双链断裂。 需要常规的高分辨率质谱学来表征 合成这些积木的中间体