EIGHT OPIATE DRUGS IN URINE BY GC & MS OF THEIR TRIMETHYLSILYL ETHER DERIVATIVES

通过 GC 检测尿液中的八种阿片类药物

• 批准号: 6118619
• 负责人: JIANBO ZENG
• 金额: $ 0.32万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118619
• 关键词: biomedical resource; drug /agent; drug adverse effect; human tissue; mental disorders; rehabilitation; spectrometry; substance abuse related disorder; technology /technique; urinary tract

Opiates are commonly abused substances, and forensic urine drug-testing for them involves an immunoassay screen and gas chromatographic/mass spectrometric (GC/MS) confirmation. There are also medical reasons to test urine for opiates, and confirmation procedures other than GC/MS are often used for medical drug-testing which are more compatible with the demands of clinical services and which identify a wider range of opiates than those in standard forensic batteries. One such procedure involves thin-layer chromatographic (TLC) analysis of opiate derivatives and can distinguish eight clinically encountered opiates, including morphine, acetylmorphine, hydromorphone, oxymorphone, codeine, dihydrocodeine, hydrocodone, and oxycodone. Medical drug-testing results are sometimes challenged by patients, causing physicians to request additional confirmation of the identified opiates. To our knowledge, no previous report examines all opiates specified above in a single GC/MS procedure, but we find that they can be distinguished by GC/MS analyses of trimethylsilyl (TMS) ether derivatives, the mass spectra of which contain prominent molecular ions. Inclusion of deuterium-labeled internal standards permits quantification of each of the eight opiates in urine. The GC/MS assay is linear over a concentration range which spans the TLC cutoff level, and coefficients of variation of 10% or less at concentrations below the TLC cutoff are achieved by for all opiates specified above except for oxymorphone and oxycodone, which exhibit coefficients of variation of 18-19%. This procedure has proved useful as a third-stage identification step for medical drug-testing specimens in which results from prior immunoassay and TLC analyses were challenged.

鸦片类药物是常见的滥用物质，法医尿液 对他们的药物测试包括免疫分析筛查和气体 色质联用(GC/MS)确认。确实有 还有检查尿液中鸦片类药物的医学原因，以及确认 除了GC/MS之外的其他程序经常被用于医学药物测试 更符合临床服务的需求，以及 它们识别的鸦片类药物比标准药物的范围更广 法医电池。一种这样的程序涉及薄层 阿片类药物及其罐头的薄层色谱分析 区分临床上遇到的八种鸦片类药物，包括吗啡， 乙酰吗啡，氢吗啡，羟吗啡，可待因，二氢可待因， 氢可酮和羟考酮。医疗药物检测结果如下 有时会受到患者的质疑，导致医生要求 对已确认的鸦片类药物的补充确认。据我们所知， 以前没有一份报告检查了上述所有阿片类药物的单一 GC/MS程序，但我们发现它们可以通过GC/MS进行区分 三甲基硅基(TMS)醚衍生物的分析、质谱分析 其中含有显著的分子离子。包括以下内容 氚标记的内标物允许对每一种 尿液中的八种鸦片类药物。GC/MS分析在 跨越TLC截止水平的浓度范围和系数 在TLC截止浓度以下的10%或更小的偏差为 通过以上规定的所有阿片类药物实现，但羟吗啡和 羟考酮，其变异系数为18-19%。这 程序已被证明是有用的第三阶段识别步骤 先前免疫化验结果的医用药物测试标本 而TLC分析受到了挑战。