EIGHT OPIATE DRUGS IN URINE BY GC & MS OF THEIR TRIMETHYLSILYL ETHER DERIVATIVES

阿片类药物是常见的滥用物质， 对他们的药物测试包括免疫测定屏幕和气体 色谱/质谱（GC/MS）确认。 有 也有医学上的原因来测试尿液中的阿片类药物， 除GC/MS外的其他程序通常用于医学药物检测 更符合临床服务的需求， 它比标准中的阿片类药物识别范围更广， 法医电池 一种这样的程序涉及薄层 阿片衍生物的薄层色谱分析， 区分八种临床上遇到的阿片类药物，包括吗啡， 乙酰吗啡，氢吗啡酮，羟吗啡酮，可待因，二氢可待因， 氢可酮和羟考酮。 医学药物测试结果是 有时会受到患者的质疑，导致医生要求 进一步确认已确定的鸦片制剂。 据我们所知， 以前的报告没有一次审查上述所有阿片剂， GC/MS方法，但我们发现它们可以区分 三甲基硅烷基（TMS）醚衍生物的分析，质谱 其中含有突出的分子离子。 列入 氘标记的内标物允许定量每种 尿液中的八种鸦片 GC/MS分析在以下范围内呈线性： 跨越TLC截止水平的浓度范围和系数 在低于TLC截止值的浓度下， 除羟吗啡酮外， 羟考酮，其表现出18 - 19%的变异系数。 这 程序已被证明是有用的第三阶段识别步骤， 先前免疫测定结果的医疗药物测试样本 TLC分析受到质疑。

Opiates are commonly abused substances, and forensic urine drug-testing for them involves an immunoassay screen and gas chromatographic/mass spectrometric (GC/MS) confirmation. There are also medical reasons to test urine for opiates, and confirmation procedures other than GC/MS are often used for medical drug-testing which are more compatible with the demands of clinical services and which identify a wider range of opiates than those in standard forensic batteries. One such procedure involves thin-layer chromatographic (TLC) analysis of opiate derivatives and can distinguish eight clinically encountered opiates, including morphine, acetylmorphine, hydromorphone, oxymorphone, codeine, dihydrocodeine, hydrocodone, and oxycodone. Medical drug-testing results are sometimes challenged by patients, causing physicians to request additional confirmation of the identified opiates. To our knowledge, no previous report examines all opiates specified above in a single GC/MS procedure, but we find that they can be distinguished by GC/MS analyses of trimethylsilyl (TMS) ether derivatives, the mass spectra of which contain prominent molecular ions. Inclusion of deuterium-labeled internal standards permits quantification of each of the eight opiates in urine. The GC/MS assay is linear over a concentration range which spans the TLC cutoff level, and coefficients of variation of 10% or less at concentrations below the TLC cutoff are achieved by for all opiates specified above except for oxymorphone and oxycodone, which exhibit coefficients of variation of 18-19%. This procedure has proved useful as a third-stage identification step for medical drug-testing specimens in which results from prior immunoassay and TLC analyses were challenged.