GLYCOLYTIC PATHWAY, REDOX STATE OF NAD(P) COUPLES & ENERGY METABOLISM IN LENS

本研究的目的是评估早期变化， 糖酵解，游离胞质NAD（P）-对的氧化还原状态， 对照组和50%组透镜中的腺嘌呤核苷酸系统 半乳糖喂养的大鼠，有可能预防这些与 醛糖还原酶抑制剂（ARI）。 实验在男性 Sprague-Dawley大鼠喂食半乳糖饮食2-14天。 水平 葡萄糖，半乳糖，糖酵解中间体，β-甘油磷酸， 用荧光分光光度法测定了100例正常人和100例正常人的苹果酸、NAD、ATP、ADP、AMP含量。 通过酶促程序，而半乳糖醇和 通过GC-MS对肌醇进行定量。抑制透镜中的糖酵解 在半乳糖喂养的大鼠中， 腺嘌呤核苷酸磷酸化。 透镜葡萄糖的早期变化 利用，NAD-对的氧化还原状态，和能量代谢， 半乳糖血症模型与糖尿病模型相似，至少 部分由醛糖还原酶所涉及的机制介导，并且可以 被醛糖还原酶抑制剂部分阻止。

The present study was aimed at evaluating early changes in glycolysis, the redox state of free cytosolic NAD(P)-couples, and the adenine nucleotide system in lens in both control and 50% galactose-fed rats, with the possibility of preventing these with an aldose reductase inhibitor (ARI). Experiments were performed on male Sprague-Dawley rats fed the galactose diet for 2-14 days. The levels of glucose, galactose, glycolytic intermediates, (-glycerophosphate, malate, NAD, ATP, ADP, AMP were assayed spectrofluorometrically in individual lenses by enzymatic procedures, while galactitol and myo-inositol were quantified by GC-MS. Depressed glycolysis in lens in galactose-fed rats is consistent with decreased NAD+/NADH and adenine nucleotide phosphorylation. Early changes in lens glucose utilization, redox state of NAD-couples, and energy metabolism in this model of galactosemia are similar to those in diabetes, are at least in part mediated by aldose reductase involved mechani sms, and can be partially prevented by an aldose reductase inhibitor.