PEPTIDE STRUCTURE & DYNAMICS
PEPTIDE STRUCTURE & DYNAMICS
批准号:
6279694
负责人:
MARINA L BENNATI
金额:
$1.18万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-05-01 至 1999-04-30
中文摘要
了解肽结构和动力学非常有趣
在化学和生物学中。 EPR方法已被用于排序
双标记肽中侧链之间的距离,从而
揭示局部折叠几何形状。 最近,位点特异性旋转的EPR
标记肽已被证明是一种出色的探针
位置相关的动力学。 尽管这些 EPR 实验
大大增加了我们对肽运动的理解,许多
重要的细节仍未解决。 特别是传统的9
GHz EPR 频谱对各向异性不是特别敏感
自旋标签运动。 本地标签的全面描述
为了澄清该条款的解释,有必要动议
位置相关的动力学并有助于确定
双标签实验中的距离。 高频 EPR 已
经证明比 9 GHz 频谱对运动更敏感
短相关时间下的各向异性。 我们获得了高
基于丙氨酸的 3K-11 螺旋肽的频率 EPR 谱图
温度从 275-305 K。 3K- I I 螺旋肽,如下所示,
在半胱氨酸上用甲硫代磺酸盐 (MTSSL) 自旋进行标记
标签。 Ac-AAAAKAAAAKCAAAKA-NH2 高频 EPR 谱图显示
对运动各向异性比低频频谱更敏感
但仍然可以根据以下内容进行简单的线形分析
运动线变窄理论。 实验模拟
使用不同的自旋各向异性运动模型的光谱
附着在肽主链上的标签链正在进行中。
英文摘要
Understanding peptide structure and dynamics is of great interest
in chemistry and biology. EPR methods have been used to rank
distances between side chains in doubly labeled peptides, thereby
revealing local folding geometry. Recently, EPR of site-specific spin
labeled peptides has been shown to be an excellent probe
of-position-dependent dynamics. Although these EPR experiments have
added considerably to our understanding of peptide motions, many
important details remain unresolved. In particular, conventional 9
GHz EPR spectra are not particularly sensitive to the anisotropy of
the spin label motion. Thorough characterization of the local label
motion is necessary in order to clarify the interpretation of the
position-dependent dynamics and to aid in the determination of
distances in double label experiments. High frequency EPR has been
demonstrated to be more sensitive than 9 GHz spectra to motional
anisotropy at short correlation times. We have obtained high
frequency EPR spectra of the alanine based 3K- 11 helical peptide at
temperatures from 275-305 K. The 3K- I I helical peptide, shown below,
is labeled at the cysteine with a methanethiosulfonate (MTSSL) spin
label. Ac-AAAAKAAAAKCAAAKA-NH2 The high frequency EPR spectra exhibit
greater sensitivity to motional anisotropy than low frequency spectra
but remain amenable to simple line shape analysis according to
motional line narrowing theory. Simulations of the experimental
spectra using different models for the anisotropic motion of the spin
label chain attached to the peptide backbone are in progress.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
DEVELOPMENT OF PULSED ELECTRON NUCLEAR DOUBLE RESONANCE (ENDOR) AT 140 GHZ
-
批准号:6355150
-
项目类别:
-
资助金额:$1.93万
-
财政年份:2000
-
负责人:MARINA L BENNATI
-
依托单位:
STRUCTURAL DETERMINATION OF PARAMAGNETIC CENTER IN PUTIDAREDOXIN
-
批准号:6355159
-
项目类别:
-
资助金额:$2.03万
-
财政年份:2000
-
负责人:MARINA L BENNATI
-
依托单位:
IDENTIFICATION OF RADICAL INTERMEDIATES DURING INACTIVATION OF CLASS I RNRS
-
批准号:6355161
-
项目类别:
-
资助金额:$2.46万
-
财政年份:2000
-
负责人:MARINA L BENNATI
-
依托单位:
NITROXIDE SIDE CHAIN DYNAMICS IN SPIN LABELED HELIX FORMING PEPTIDE
-
批准号:6355148
-
项目类别:
-
资助金额:$1.82万
-
财政年份:2000
-
负责人:MARINA L BENNATI
-
依托单位:
APPLICATION OF 140 GHZ PULSED ENDOR TO CLASS I RNRS
-
批准号:6355160
-
项目类别:
-
资助金额:$2.14万
-
财政年份:2000
-
负责人:MARINA L BENNATI
-
依托单位:
STRUCTURAL DETERMINATION OF PARAMAGNETIC CENTER IN PUTIDAREDOXIN
-
批准号:6118646
-
项目类别:
-
资助金额:$2.03万
-
财政年份:1999
-
负责人:MARINA L BENNATI
-
依托单位:
NITROXIDE SIDE CHAIN DYNAMICS IN SPIN LABELED HELIX FORMING PEPTIDE
-
批准号:6118650
-
项目类别:
-
资助金额:$1.82万
-
财政年份:1999
-
负责人:MARINA L BENNATI
-
依托单位:
APPLICATION OF 140 GHZ PULSED ENDOR TO CLASS I RNRS
-
批准号:6118647
-
项目类别:
-
资助金额:$2.14万
-
财政年份:1999
-
负责人:MARINA L BENNATI
-
依托单位:
IDENTIFICATION OF RADICAL INTERMEDIATES DURING INACTIVATION OF CLASS I RNRS
-
批准号:6118648
-
项目类别:
-
资助金额:$2.46万
-
财政年份:1999
-
负责人:MARINA L BENNATI
-
依托单位:
DEVELOPMENT OF PULSED ELECTRON NUCLEAR DOUBLE RESONANCE (ENDOR) AT 140 GHZ
-
批准号:6118649
-
项目类别:
-
资助金额:$1.93万
-
财政年份:1999
-
负责人:MARINA L BENNATI
-
依托单位:
HIGH FREQUENCY ELECTRON NUCLEAR DOUBLE RESONANCE
-
批准号:6279715
-
项目类别:
-
资助金额:$1.18万
-
财政年份:1998
-
负责人:MARINA L BENNATI
-
依托单位:
A 139.5 GHZ PULSED EPR AND ENDOR SPECTROMETER
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批准号:6279714
-
项目类别:
-
资助金额:$2.37万
-
财政年份:1998
-
负责人:MARINA L BENNATI
-
依托单位: