VERY HIGH RESOLUTION XRAY DATA COLLECTION ON DIMERIZATION DOMAIN OF HNF 1A

HNF 1A 二聚域的极高分辨率 X 射线数据采集

• 批准号: 6119503
• 负责人: JAMES A ENDRIZZI
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119503
• 关键词: biomaterials; proteins; structural biology

Collection of very high resolution data on HNF-p1 would enable us to solve the crystal structure using direct methods. This will provide a detailed look at the HNF-1 dimerization motif, which has been proposed to exist as a kinked helix pair or a four-helix bundle. Comparison with structures of free DCoH and DCoH/HNF-p1 complex would allow us to define interactions that mediate recognition and to characterize any conformational changes that may help stimulate gene expression.

在HNF-p1上收集非常高分辨率的数据将使我们能够 用直接法求解晶体结构。这将是 详细了解HNF-1的二聚化基序，它具有 已经被提出以扭结的螺旋对或四螺旋束状存在。 游离DCoH和DCoH/HNF-p1复合体的结构比较 允许我们定义中介识别的交互，并 描述任何可能有助于刺激基因的构象变化 表情。