ADENOSINE KINETICS IN CULTURED ENDOTHELIAL CELLS OF HEART

培养的心脏内皮细胞中的腺苷动力学

• 批准号: 6119784
• 负责人: ANDREAS DEUSSEN
• 金额: $ 1.35万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-16 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119784
• 关键词: Mammalia; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical resource; human tissue; model design /development; radiography; technology /technique

Coronary endothelial cells not only produce vasoactive adenosine but also represent a metabolic barrier for this nucleoside. The purpose of the present experiments was to improve our understanding of the importance of the endothelial cells for the adjustment of the vascular adenosine concentration. Coronary endothelial cells of guinea pig heart grown to confluence on solid microcarrier beads were superfused in a chromatography column at 2 ml/min. Endothelial cell volume was determined with the 3-O-methyl-D-glucose method. Under control conditions the adenosine release in the coronary effluent perfusate averaged 0.6 pmol min-1 l-1. Measurement of the column adenosine release during infusion of specific inhibitors of the various pathways of adenosine metabolism and transport were used to assess intra- and extracellular adenosine production rates (4.5 vs. 1.1 pmol min-1 l-1). Assessment of the column outflow concentration during variation of the column inflow concentration permitted asses sment of the surface adenosine concentration of coronary endothelial cells which was found to range from 4 to 9 nM. These experimental data provide a basis for a model analysis in order to quantify the contribution of adenosine metabolism and transport of coronary endothelial cells to global cardiac adenosine production and to assess its influence on the capillary and interstitial adenosine concentration on the example of the guinea pig heart.

冠状动脉内皮细胞不仅产生血管活性腺苷 但也代表了该核苷的代谢屏障。 这 本实验的目的是提高我们对 内皮细胞对于调节的重要性 血管腺苷浓度。 冠状动脉内皮细胞 豚鼠心脏在固体微载体珠上生长至汇合 以 2 ml/min 的速度注入色谱柱。 内皮细胞 体积用3-O-甲基-D-葡萄糖法测定。 在下面 控制冠状动脉流出物中腺苷释放的条件 灌注液平均为 0.6 pmol min-1 l-1。 色谱柱的测量 输注特定抑制剂期间腺苷释放 腺苷代谢和运输的多种途径被用来 评估细胞内和细胞外腺苷的生产率（4.5 vs. 1.1 pmol min-1 l-1)。 柱流出浓度的评估 在塔流入浓度变化期间允许的评估 冠状动脉内皮表面腺苷浓度的测定 细胞被发现范围为 4 至 9 nM。 这些实验性的 数据为模型分析提供基础，以量化 腺苷代谢和冠状动脉转运的贡献 内皮细胞对整体心脏腺苷产生的影响并评估 对毛细血管和间质腺苷的影响 专注于豚鼠心脏的例子。