BACTERIOPHAGE LAMBDA LYSOZYME BY MULTI ANOMALOUS DISPERSION METHOD

多重异常分散法制备噬菌体 Lambda 溶菌酶

• 批准号: 6120472
• 负责人: ALBERT M BERGHUIS
• 金额: $ 0.02万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120472
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

The following three projects have benefited from experiments performed at the Cornell High Energy Synchrotron Source. -Lambda phage lysozyme lysozyme from lambda phage, like other lysozymes, is capable of hydrolyzing bacterial cell-walls. However, the mechanism by which this hydrolysis is accomplished differs dramatically from that of other lysozymes (e.g. T4 - and hen egg-white lysozyme). We have recently solved the structure of the enzyme in the presence of an inhibitor to 2.7 Angstrom, by molecular replacement. The structure provides insight into the unique mechanism employed by this enzyme for hydrolyzing the peptidoglycan layer of bacterial cell-walls. - fungal homoserine dehydrogenase Homoserine dehydrogenase from fungal sources has been shown to be an excellent target for antifungal agents. Since this 370 residue enzyme lacksany methionines, we have pursued the multiple isomorphous replacement method for determining the three-dimensional structure. Optimized anomalous derivative data collected at the CHESS F2 beam-line has resulted in a partial tracing of the structure. Unfortunately, severe non-isomorphism between crystals has hampered rapid progress. - AAC(6')-Ii AAC(6')-Ii is a bacterial enzyme responsible for bacterial resistance against aminoglycoside antibiotics. Two MAD datasets of a selinomethionine derivative of this enzyme were collected at the CHESS F2 beam-line. We have successfully solved the structure, and are currently refining the atomic model. The significance of this structure determination considering the problem of antibiotic resistance is obvious.

以下三个项目已从试验中获益 在康奈尔高能同步加速器源上进行。 -Lambda 噬菌体溶菌酶来自λ噬菌体的溶菌酶，像其他溶菌酶一样， 能够水解细菌细胞壁。 然而，机制 完成这种水解的方法与 其他溶菌酶（例如T4 -和鸡蛋清溶菌酶）的活性。 我们 最近已经解决了酶的结构，在存在一个 抑制剂到2.7埃，通过分子置换。 结构 提供了对这种酶所采用的独特机制的见解， 水解细菌细胞壁的肽聚糖层。 - 真菌 真菌来源高丝氨酸脱氢酶 已被证明是抗真菌剂的极好靶点。 以来 这种370个残基的酶缺乏任何甲硫氨酸， 多重同晶置换法 三维结构。 优化异常导数数据 在CHESS F2光束线处收集的数据导致了部分跟踪 的结构。 不幸的是， 晶体阻碍了快速发展。 - AAC（6 '）-Ii AAC（6'）-Ii是 负责细菌抵抗的细菌酶 氨基糖苷类抗生素 硒代蛋氨酸的两个MAD数据集 在CHESS F2光束线处收集该酶的衍生物。 我们已经成功地解决了结构，目前正在完善 原子模型。 这种结构测定的意义 抗生素耐药性的问题是显而易见的。