ISOLATED AMPHIBIAN RETINA

孤立的两栖动物视网膜

• 批准号: 6122342
• 负责人: THOMAS G EBREY
• 金额: $ 0.81万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-10 至 2000-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122342
• 关键词: Chordata; bioimaging /biomedical imaging; biomedical resource; carbohydrates; eye; human tissue; magnetic resonance imaging; nuclear magnetic resonance spectroscopy; technology /technique

31P and 1H spectra have been obtained on intact isolated and superfused toad retinae. Up to eight retinae are studied in a commercial probe and a single retina is studied in a purpose-built small-sample spectroscopy probe. Adequacy of tissue maintenance conditions has been demonstrated. Differences in phosphorus metabolite concentrations have been observed under conditions of light and dark adaptation. Cyclic GMP has been demonstrated in the 31P NMR spectrum of retina.The Pa/Pi ratio increases and [La] decreases in high calcium solution, a useful finding since calcium is thought to be involved in light adaptation.When the Na/K pump is blocked with ouabain in low calcium, both Pa/Pi and lactate decrease, suggesting differing functional roles for anaerobic and aerobic glycolysis.

~（31）P和~ 1H能谱已在完整的分离样品上获得， 灌注蟾蜍视网膜。 多达八个视网膜被研究， 商业探针和一个单一的视网膜是在一个专门建造的研究 小样品光谱探针。 组织维护的准确性 条件得到了证明。 磷的差异 在光照条件下观察到代谢物浓度 黑暗的适应 环GMP已在31 P NMR中得到证实 视网膜的光谱。Pa/Pi比值增加，[La]降低， 高钙溶液，一个有用的发现，因为钙被认为是 参与光适应。当Na/K泵被阻断时， 哇巴因在低钙时，Pa/Pi和乳酸均降低，提示 厌氧和有氧糖酵解的不同功能作用。