ISOLATED AMPHIBIAN RETINA

孤立的两栖动物视网膜

• 批准号: 6282377
• 负责人: THOMAS G EBREY
• 金额: $ 0.94万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-15 至 1999-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282377
• 关键词: Chordata; biomedical resource; carbohydrates; eye; human tissue; magnetic resonance imaging; nuclear magnetic resonance spectroscopy; technology /technique

31P and 1H spectra have been obtained on intact isolated and superfused toad retinae. Up to eight retinae are studied in a commercial probe and a single retina is studied in a purpose-built small-sample spectroscopy probe. Adequacy of tissue maintenance conditions has been demonstrated. Differences in phosphorus metabolite concentrations have been observed under conditions of light and dark adaptation. Cyclic GMP has been demonstrated in the 31P NMR spectrum of retina.The Pa/Pi ratio increases and [La] decreases in high calcium solution, a useful finding since calcium is thought to be involved in light adaptation.When the Na/K pump is blocked with ouabain in low calcium, both Pa/Pi and lactate decrease, suggesting differing functional roles for anaerobic and aerobic glycolysis.

获得了完整的分离样品的~(31)P和~1H谱 超融合的青蛙视网膜。在一项研究中研究了多达8个视网膜 商业探头和单个视网膜在专门建造的 小样本光谱探头。组织维护的充分性 条件已经得到了证明。磷的差异 在光照条件下观察到了代谢物的浓度。 和黑暗适应。环状GMP已在31P核磁共振中得到证实 视网膜的光谱分析。随着年龄的增加，PA/PI比值增大，[La]降低 高钙溶液，一个有用的发现，因为钙被认为是 参与光适应。当Na/K泵被 哇巴因在低钙时，PA/PI和乳酸均下降，提示 厌氧糖酵解和有氧糖酵解的不同作用。