SEQUENCE ANALYSIS OF ARABIDOPSIS THALIANA MICROTUBULE ASSOCIATED PROTEIN CDNAS

微管相关蛋白(MAP)具有重要的生物学意义 因为它们在维护结构性组织方面很重要 和微管细胞球蛋白的稳定性。拟议的研究 旨在分离高等植物中编码图谱的基因， 特别强调最终确定这些 蛋白质在植物细胞中发挥作用。属于一组的蛋白质 包括MAP2、tau和MAP4在内的哺乳动物图谱都显示出很高的 羧基末端的蛋白质序列保守度 用来结合微管的结构域。我们假设这些 保守序列也存在于植物MAP蛋白中。我们的 初步数据显示编码该基因的c DNA与 小鼠MAP2保守区和拟南芥基因组DNA 萨利安支持这一假设。我们建议分离全长 将拟南芥的cdna克隆作图。一种确认是否或 不是这些cDNA代表了真正的图谱基因将会比较它们的 核苷酸和衍生氨基酸序列与已知的核苷酸和氨基酸序列 哺乳动物地图。我们建议使用威斯康星大学的GCG 用于这些序列比较的序列分析程序。这和 该项目的其他方面将扩展细胞生物学 了解植物细胞骨架及其在细胞内的功能。 它将提供关于一组蛋白质的重要信息，其中 人们对它们的功能有很多猜测，但很少有文献记载 在植物发育的关键时期。

Microtubule associated proteins (MAPs) are of biological interest because they are important inmaintaining the structural organization and stability of the microtubular cytoskelton. The proposed research is aimed at isolating genes which encode MAPs in higher plants, with a particular emphasis upon the eventual determination of how these proteins function in plant cells. The proteins belonging to one group of mammalian MAPs, which includes MAP2, tau an MAP4, all show a high degree of protein sequence conservation within a carboxy-terminal domain that serves to bind microtubles. We hypothesize that these conserved sequences are also present in plant MAP proteins. Our preliminary data showing hybridization between a cDNA encoding the conserved region of mouse MAP2 and genomic DNA from Arabidopsis thalian supports this hypothesis. We propose to isolate full-length MAP cDNA clones from Arabidopsis. One means of confirming whether or not these cDNAs represent true MAP genes will be to compare their nucleotide and derived amino acid sequences with those of known mammalian MAPs. We propose to use the University of Wisconsin GCG sequence analysis programs for these sequences comparisons. This and other aspects of this project will extend the cellular biological knowledge of the plant cytoskeleton and its functions within the cell. It will provide important information about a group of proteins where there is much speculation but little documentation of their functions during critical times in plant development.