SEQUENCE ANALYSIS OF ARABIDOPSIS THALIANA MICROTUBULE ASSOCIATED PROTEIN CDNAS

微管相关蛋白是一类具有生物学意义的蛋白质 because they are important重要inmaintaining维持the structural结构organization组织 和微管胞浆的稳定性。 拟议研究 目的是分离高等植物中编码MAPs的基因， 特别强调最终确定这些 蛋白质在植物细胞中起作用。 属于一组的蛋白质 包括MAP2、tau和MAP4在内的哺乳动物MAPs，都显示出高表达。 羧基末端内蛋白质序列保守程度 用于结合微管的结构域。 我们假设这些 保守序列也存在于植物MAP蛋白中。 我们 初步数据显示了编码 小鼠MAP2保守区和拟南芥基因组DNA 塔利安支持这一假设。 我们建议分离全长 来自拟南芥的MAP cDNA克隆。 确认是否或 这些cDNA并不代表真正的MAP基因， 核苷酸和衍生的氨基酸序列与已知的核苷酸和衍生的氨基酸序列相比， 哺乳动物的地图 我们建议使用威斯康星州大学的GCG 用于这些序列比较的序列分析程序。 这个和 该项目的其他方面将扩展细胞生物学 了解植物细胞骨架及其在细胞内的功能。 它将提供关于一组蛋白质的重要信息， 关于它们的功能有很多猜测，但很少有文献记载 在植物发育的关键时期。

Microtubule associated proteins (MAPs) are of biological interest because they are important inmaintaining the structural organization and stability of the microtubular cytoskelton. The proposed research is aimed at isolating genes which encode MAPs in higher plants, with a particular emphasis upon the eventual determination of how these proteins function in plant cells. The proteins belonging to one group of mammalian MAPs, which includes MAP2, tau an MAP4, all show a high degree of protein sequence conservation within a carboxy-terminal domain that serves to bind microtubles. We hypothesize that these conserved sequences are also present in plant MAP proteins. Our preliminary data showing hybridization between a cDNA encoding the conserved region of mouse MAP2 and genomic DNA from Arabidopsis thalian supports this hypothesis. We propose to isolate full-length MAP cDNA clones from Arabidopsis. One means of confirming whether or not these cDNAs represent true MAP genes will be to compare their nucleotide and derived amino acid sequences with those of known mammalian MAPs. We propose to use the University of Wisconsin GCG sequence analysis programs for these sequences comparisons. This and other aspects of this project will extend the cellular biological knowledge of the plant cytoskeleton and its functions within the cell. It will provide important information about a group of proteins where there is much speculation but little documentation of their functions during critical times in plant development.