MRC WIMM bid - Maintain and expand capabilities of the MRC WIMM Wolfson Imaging Centre with a Zeiss LSM 980 confocal microscope.

广泛的标本（如固定或活细胞和组织、类器官、球体和胚胎，甚至小动物如小鼠和斑马鱼）的光显微成像已经成为跨学科生物医学研究的一个组成部分。在整个牛津，这种成像需求通过一系列多用户核心成像设施得到最有效的满足，工作人员为各种先进的显微镜设备提供专家支持和培训。MRC Weatherall分子医学研究所（WIMM）的沃尔夫森成像中心成立于2012年，是MRC WIMM的500多名研究人员唯一的先进光学显微镜设施，同时也为JR医院以及牛津大学其他设施不提供的专业知识（例如STED或FLIM）的用户提供服务。MRC WIMM Wolfson成像中心提供12台先进的光学显微镜，并为来自RDM， NDM，肿瘤学，生物化学，DPAG，药理学，植物科学，邓恩学院和肯尼迪研究所的60多个独立研究小组的230多名注册用户提供服务。月平均累计使用用户大于60人，月累计使用时间约1200小时。自2012年以来，依靠中心设备发表了100多篇论文，包括《自然》、《自然免疫学》、《自然细胞生物学》、《血液》等期刊。中心最常用的仪器是共聚焦显微镜，每月使用800-900小时。该应用程序的目的是解决需要更换该设施最常用的仪器之一，蔡司LSM780（在过去5年中有145个个人用户，平均每月使用185小时），该仪器已变得不可靠并已达到其使用期限。我们正在寻求用更新和增强的型号（LSM980）取代这种经常使用的仪器，以保持满足WIMM成像中心的高需求，同时增加新的成像功能。

Light-microscopic imaging of a broad range of specimens (like fixed or live cells and tissues, organoids, spheroids and embryos, even small animals like mouse and zebrafish) has become an integral part of interdisciplinary biomedical research. Across Oxford this imaging demand is most efficiently met by a range of multi-user core imaging facilities with staff that provide expert support and training for a broad range of advanced microscope equipment. The Wolfson Imaging Centre at the MRC Weatherall Institute of Molecular Medicine (WIMM) was set up in 2012 and serves as the sole advanced light microscope facility for over 500 researchers at the MRC WIMM, but also for those from the JR Hospital site as well as users across the University who seek an expertise (e.g. STED or FLIM) that is not provided at other facilities across Oxford.The MRC WIMM Wolfson Imaging Centre provides 12 advanced light microscopes and serves in excess of 230 registered users from greater than 60 independent research groups from RDM, NDM, Oncology, Biochemistry, DPAG, Pharmacology, Plant Sciences, Dunn School, and Kennedy Institute. The average accumulated monthly use is greater than 60 users for an accumulated monthly time of about 1,200 hours. Since 2012 more than 100 papers that relied on Centre equipment have been published, including in journals such as Nature, Nature Immunology, Nature Cell Biology, Blood, and more.The most frequently used instruments at the Centre are the confocal microscopes with 800-900 hours per month. The aim of this application is to address the need to replace one of the facility's most used instruments, a Zeiss LSM780 (145 individual users in the last 5 years, and used at 185 hours/month on average), which has become unreliable and has reached the end of its use. We are seeking to replace this much used instrument with a newer and enhanced model (LSM980) to keep meeting the high demand at the WIMM Imaging Centre, while also adding new imaging capabilities.