MRC WIMM bid - Maintain and expand capabilities of the MRC WIMM Wolfson Imaging Centre with a Zeiss LSM 980 confocal microscope.

MRC WIMM 投标 - 使用蔡司 LSM 980 共焦显微镜维护和扩展 MRC WIMM Wolfson 成像中心的功能。

• 批准号: MR/X012743/1
• 负责人: Jana Koth
• 金额: $ 77.33万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX012743%2F1
• 关键词: MRC; WIMM; bid; Maintain; expand

Light-microscopic imaging of a broad range of specimens (like fixed or live cells and tissues, organoids, spheroids and embryos, even small animals like mouse and zebrafish) has become an integral part of interdisciplinary biomedical research. Across Oxford this imaging demand is most efficiently met by a range of multi-user core imaging facilities with staff that provide expert support and training for a broad range of advanced microscope equipment. The Wolfson Imaging Centre at the MRC Weatherall Institute of Molecular Medicine (WIMM) was set up in 2012 and serves as the sole advanced light microscope facility for over 500 researchers at the MRC WIMM, but also for those from the JR Hospital site as well as users across the University who seek an expertise (e.g. STED or FLIM) that is not provided at other facilities across Oxford.The MRC WIMM Wolfson Imaging Centre provides 12 advanced light microscopes and serves in excess of 230 registered users from greater than 60 independent research groups from RDM, NDM, Oncology, Biochemistry, DPAG, Pharmacology, Plant Sciences, Dunn School, and Kennedy Institute. The average accumulated monthly use is greater than 60 users for an accumulated monthly time of about 1,200 hours. Since 2012 more than 100 papers that relied on Centre equipment have been published, including in journals such as Nature, Nature Immunology, Nature Cell Biology, Blood, and more.The most frequently used instruments at the Centre are the confocal microscopes with 800-900 hours per month. The aim of this application is to address the need to replace one of the facility's most used instruments, a Zeiss LSM780 (145 individual users in the last 5 years, and used at 185 hours/month on average), which has become unreliable and has reached the end of its use. We are seeking to replace this much used instrument with a newer and enhanced model (LSM980) to keep meeting the high demand at the WIMM Imaging Centre, while also adding new imaging capabilities.

广泛的标本（如固定或活细胞和组织，类器官，球体和胚胎，甚至小动物，如小鼠和斑马鱼）的光学显微镜成像已成为跨学科生物医学研究的一个组成部分。在牛津，这种成像需求最有效地满足了一系列多用户核心成像设施，其工作人员为各种先进的显微镜设备提供专家支持和培训。MRC Weatherall分子医学研究所（WIMM）的Wolfson成像中心成立于2012年，是MRC WIMM 500多名研究人员的唯一先进光学显微镜设施，也适用于JR医院网站以及大学内寻求专业知识的用户（例如STED或FLIM）MRC WIMM Wolfson成像中心提供12台先进的光学显微镜，为来自60多个独立研究小组的230多名注册用户提供服务。来自RDM，NDM，肿瘤学，生物化学，DPAG，药理学，植物科学，邓恩学校和肯尼迪研究所。平均每月累计使用超过60个用户，每月累计使用时间约1,200小时。自2012年以来，已经发表了100多篇依赖于中心设备的论文，包括在Nature，Nature Immunology，Nature Cell Biology，Blood等期刊上。中心最常用的仪器是共聚焦显微镜，每月800-900小时。该申请的目的是解决更换该设施最常用的仪器之一蔡司LSM 780的需求（在过去5年中有145名个人用户，平均每月使用185小时），该仪器已变得不可靠并已达到使用期限。我们正在寻求用更新和增强的型号（LSM 980）取代这种使用频繁的仪器，以继续满足WIMM成像中心的高需求，同时还增加了新的成像功能。