Automated Peptide Synthesiser

自动化肽合成仪

• 批准号: MR/X013545/1
• 负责人: Sylvain Ladame
• 金额: $ 24.41万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX013545%2F1
• 关键词: Automated; Peptide; Synthesiser

Peptides are short chains of amino acids that can be organised in large and complex structures with uniquely desirable chemical and biological properties. For these reasons, peptides or peptide derivatives have been extensively used in biomedical research as probes (to investigate fundamental biological processes in vitro or in cells); as drugs (being main constituents of drugs and vaccines), or as implants with unique (e.g., adhesive) properties. Researchers across departments at Imperial College develop and use peptides with specific applications in all these areas. Example of application include:(1) The development of new tools to help improve the way diseases, such as cancer, are diagnosed and then treated. (2) The development of new materials such as adhesives that can help repair failing hearts, bone tissue matrices. (3) The development of novel devices for drug delivery, or specific applications such as minimising risks during bypass surgery.The requested automated peptide synthesiser, PurePep Chorus, will enable the parallel synthesis of up to six peptides simultaneously. As such It will free up valuable staff time by replacing the laborious and time-consuming procedure of manual peptide synthesis. Importantly it will alleviate the health risks associated with the process of manual peptide synthesis due to the exposure to allergens and toxic chemicals. The non-availability of an in-house state-of-the art peptide synthesiser is a key bottle neck in the progression and expansion of research at Imperial, examples of which are described above. The PurePep Chorus will be housed in the managed Bionanofabrication facility in the Sir Michael Uren Hub at White City and will be accessible to academics across Imperial College as well as external academic and industrial users.

肽是氨基酸的短链，可以组织成具有独特期望的化学和生物学性质的大而复杂的结构。由于这些原因，肽或肽衍生物已广泛用于生物医学研究中作为探针（以研究体外或细胞中的基本生物过程）;作为药物（作为药物和疫苗的主要成分）或作为具有独特生物活性的植入物（例如，粘合剂）性能。帝国理工学院各部门的研究人员开发和使用在所有这些领域具有特定应用的肽。应用的例子包括：（1）开发新的工具，以帮助改善疾病，如癌症，诊断和治疗的方式。(2)开发新材料，如粘合剂，可以帮助修复衰竭的心脏，骨组织基质。(3)开发用于药物输送的新型设备，或特定应用，如最大限度地降低旁路手术期间的风险。所要求的自动化肽合成仪PurePep Chorus将能够同时并行合成多达六种肽。因此，它将通过取代手工肽合成的费力且耗时的程序来节省宝贵的工作人员时间。重要的是，它将减轻由于暴露于过敏原和有毒化学物质而与手动肽合成过程相关的健康风险。内部最先进的肽合成仪的不可用性是Imperial研究进展和扩展的关键瓶颈，其示例如上所述。PurePep合唱团将被安置在白城迈克尔·乌伦爵士中心的生物纳米实验室设施内，帝国理工学院的学者以及外部学术和工业用户都可以使用。