FUNCTIONAL ROLE OF ESAM IN VASCULAR DEVELOPMENT

ESAM 在血管发育中的功能作用

• 批准号: 6208604
• 负责人: EUGENE YANG
• 金额: $ 3.92万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6208604
• 关键词: angiogenesis; cell adhesion molecules; cell differentiation; gene targeting; genetically modified animals; laboratory mouse; vascular endothelium

The purpose of this research is to gain further insight into the molecular mechanisms that are involved with blood vessel formation. Blood vessel formation is not only critical for normal development, but plays an essential role in the pathogenesis of many diseases. The molecular basis of this complex process has been meticulously characterized over the past few decades. Angiogenesis and vasculogenesis are both essential components of blood vessel formation. These processes require elaborate interactions between cell adhesion molecules, growth factors, extracellular matrix proteins, and intracellular signaling pathways. Adhesion molecules have been found to play a pivotal role in morphogenesis, organogenesis, and vasculogenesis. Recently, a novel immunoglobulin-type adhesion molecule named endothelial cell-specific adhesion molecule (ESAM) was cloned. ESAM expression is localized to endothelial cells and may play a fundamental role in endothelial cell development. Studies are proposed to characterize the role of ESAM in blood vessel formation. To evaluate the in vivo role of this molecule, mice lacking functional ESAM will be produced. In vitro and animal studies will be performed to determine whether ESAM plays a role in angiogenesis.

这项研究的目的是进一步了解与血管形成有关的分子机制。血管形成不仅对正常发育至关重要，而且在许多疾病的发病机制中起着至关重要的作用。在过去的几十年里，这一复杂过程的分子基础已经得到了细致的表征。血管生成和血管生成都是血管形成的重要组成部分。这些过程需要细胞黏附分子、生长因子、细胞外基质蛋白和细胞内信号通路之间复杂的相互作用。黏附分子已被发现在形态发生、器官发生和血管发生中起着关键作用。最近，一种新的免疫球蛋白型黏附分子--内皮细胞特异性黏附分子(ESAM)被克隆。ESAM表达定位于内皮细胞，可能在内皮细胞发育过程中发挥重要作用。有人建议研究ESAM在血管形成中的作用。为了评估该分子在体内的作用，将产生功能缺失的ESAM小鼠。将进行体外和动物研究，以确定ESAM是否在血管生成中发挥作用。