STRUCTURE/FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
STRUCTURE/FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
批准号:
6180259
负责人:
VICTOR L DAVIDSON
金额:
$24.26万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-08-01 至 2001-07-31
关键词:
X ray crystallography active sites amine oxidoreductase azurin biophysics chemical binding chemical kinetics cofactor cytochrome c electron nuclear double resonance spectroscopy electron spin resonance spectroscopy electron transport enzyme biosynthesis enzyme complex enzyme mechanism enzyme structure enzyme substrate nuclear magnetic resonance spectroscopy protein protein interaction quinones respiratory enzyme semiquinone site directed mutagenesis tryptophan
中文摘要
描述:该项目的目标是定义
部分藜麦蛋白脱氢酶的催化和电子转移(ET)
以及它们的生理蛋白质氧化还原伙伴。这些蛋白质正被用于
开发和检验关于(I)蛋白质结合的作用的假说
催化和内质网中的醌辅助因子,(II)长程作用机理
蛋白质间ET反应,以及(Iii)稳定特异性的因子。
促进分子间ET的可溶性蛋白质之间的相互作用。
生理性ET反应的研究在很大程度上局限于
结构信息难以确定的整体膜蛋白
获取。藜麦蛋白脱氢酶是可溶的,有两个相对的
独特而有趣的特性。它们利用酶结合的苯醌作为
假体基团,对于电子受体,他们使用其他蛋白质
而不是O2或吡啶核苷酸。我们正在描述催化反应
藜麦蛋白的作用机制及其ET动力学模型的建立
反应,并确定这些蛋白质的晶体结构
具有生理氧化还原伙伴的复合体。这使得我们能够关联
结构信息与我们的研究结果。这样做的一个主要目的是
建议使用定点突变来检验假设,这些假设
关于催化、蛋白质-蛋白质机制的研究进展
交互作用和远程ET。对影响因素的解释
藜麦蛋白及其受体之间的蛋白质-蛋白质相互作用
电子受体将提供对蛋白质-蛋白质过程的洞察
识别是广泛的生物现象所共有的。长
Range ET是生物能量现象的中心过程,如
呼吸作用和中间代谢的某些反应。
长程间质蛋白作用机制和途径的研究进展
ET将使我们更好地理解这些基本过程
分子水平。
英文摘要
DESCRIPTION: The objective of this project is to define mechanisms of
catalysis and electron transfer (ET) by selected quinoprotein dehydrogenases
and their physiologic protein redox partners. These proteins are being used
to develop and test hypotheses concerning (I) the roles of protein-bound
quinone cofactors in catalysis and ET, (ii) mechanisms of long range
interprotein ET reactions, and (iii) factors which stabilize the specific
interactions between soluble proteins that facilitate intermolecular ET.
study of physiologic ET reactions has for the most part been limited to
integral membrane proteins for which structural information is difficult to
obtain. Quinoprotein dehydrogenases are soluble and have two relatively
unique and interesting properties. They utilize enzyme-bound quinones as
prosthetic groups, and for electron acceptors they use other proteins rather
than O2 or pyridine nucleotides. We are characterizing catalytic reaction
mechanisms of the quinoproteins, developing kinetic models for their ET
reactions, and determining crystal structures of these proteins free and in
complex with physiologic redox partners. This allows us to correlate
structural information with the results of our studies. A major aim of this
proposal is to use site-directed mutagenesis to test hypothesis which have
been developed concerning mechanisms of catalysis, protein-protein
interaction and long range ET. Elucidation of factors which influence
specific protein-protein interactions between quinoproteins and their
electron acceptors will provide insight into the process of protein-protein
recognition which is common to a wide range of biologic phenomena. Long
range ET is a process which is central to bioenergetic phenomena such as
respiration and certain reactions of intermediary metabolism.
Characterization of the mechanisms and pathways of long range interprotein
ET will allow us to better understand these fundamental processes at the
molecular level.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Mechanisms of Catalysis and Cofactor Biosynthesis of Redox Enzymes with Unusual Cofactors
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批准号:10544716
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项目类别:
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资助金额:$40.12万
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财政年份:2019
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负责人:VICTOR L DAVIDSON
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依托单位:
Mechanisms of Catalysis and Cofactor Biosynthesis of Redox Enzymes with Unusual Cofactors
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批准号:10320021
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项目类别:
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资助金额:$40.13万
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财政年份:2019
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负责人:VICTOR L DAVIDSON
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依托单位:
STRUCTURE-FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
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批准号:2180920
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项目类别:
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资助金额:$19.66万
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财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
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批准号:8309196
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项目类别:
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资助金额:$31.98万
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财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
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批准号:8618284
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项目类别:
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资助金额:$36.5万
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财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
STRUCTURE-FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
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批准号:3299786
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项目类别:
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资助金额:$19.32万
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财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
-
批准号:8120827
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项目类别:
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资助金额:$31.98万
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财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
STRUCTURE-FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
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批准号:2180921
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项目类别:
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资助金额:$20.52万
-
财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
STRUCTURE FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
-
批准号:3299790
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项目类别:
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资助金额:$19.68万
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财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
STRUCTURE FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
-
批准号:3299785
-
项目类别:
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资助金额:$18.2万
-
财政年份:1988
-
负责人:VICTOR L DAVIDSON
-
依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
-
批准号:6603829
-
项目类别:
-
资助金额:$29.9万
-
财政年份:1988
-
负责人:VICTOR L DAVIDSON
-
依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
-
批准号:7471387
-
项目类别:
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资助金额:$30.31万
-
财政年份:1988
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负责人:VICTOR L DAVIDSON
-
依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
-
批准号:8516044
-
项目类别:
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资助金额:$30.86万
-
财政年份:1988
-
负责人:VICTOR L DAVIDSON
-
依托单位:
STRUCTURE-FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
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批准号:2180922
-
项目类别:
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资助金额:$21.4万
-
财政年份:1988
-
负责人:VICTOR L DAVIDSON
-
依托单位:
STRUCTURE FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
-
批准号:3299787
-
项目类别:
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资助金额:$17.83万
-
财政年份:1988
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负责人:VICTOR L DAVIDSON
-
依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
-
批准号:6780902
-
项目类别:
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资助金额:$29.9万
-
财政年份:1988
-
负责人:VICTOR L DAVIDSON
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依托单位:
STRUCTURE/FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
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批准号:6018757
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项目类别:
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资助金额:$23.55万
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财政年份:1988
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负责人:VICTOR L DAVIDSON
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依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
-
批准号:6525613
-
项目类别:
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资助金额:$29.9万
-
财政年份:1988
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负责人:VICTOR L DAVIDSON
-
依托单位:
Structure Function & Biosynthesis of Respiratory Enzymes
-
批准号:9318558
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项目类别:
-
资助金额:$36.5万
-
财政年份:1988
-
负责人:VICTOR L DAVIDSON
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依托单位:
STRUCTURE FUNCTION & BIOSYNTHESIS OF RESPIRATORY ENZYMES
-
批准号:3299789
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项目类别:
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资助金额:$18.92万
-
财政年份:1988
-
负责人:VICTOR L DAVIDSON
-
依托单位:
海外基金