MOLECULAR GENETIC ANALYSIS OF THE CYTOSKELETON

细胞骨架的分子遗传学分析

• 批准号: 6179617
• 负责人: DAVID A KNECHT
• 金额: $ 21.96万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6179617
• 关键词: Dictyostelium; actin binding protein; actins; cell adhesion; cell motility; crosslink; cytoskeleton; image processing; immunofluorescence technique; molecular cloning; molecular genetics; polymerase chain reaction; protein biosynthesis; protein structure function; tropomyosin

DESCRIPTION: The applicant will use molecular genetic, imaging and biophysical techniques to determine how the actin filament network is formed and shaped by actin cross-linking proteins. Mutant cell lines which contain alterations in genes coding for the actin binding proteins ABP-120, ABP-240 and a-actinin as well as myosin II will be studied. Several novel in vitro and in vivo assays will be used to assess the consequences of these alterations in the cytoskeleton.

描述：申请人将使用分子遗传学、成像和 生物物理技术来确定肌动蛋白丝网络是如何形成的 并由肌动蛋白交联蛋白形成。 含有以下物质的突变细胞系 编码肌动蛋白结合蛋白ABP-120、ABP-240的基因改变 和α-辅肌动蛋白以及肌球蛋白II将被研究。 几种新型体外 体内试验将用于评估这些结果， 细胞骨架的改变。