MOLECULAR GENETICS OF DEVELOPMENT IN DICTYOSTELIUM
盘基网菌发育的分子遗传学
基本信息
- 批准号:3298322
- 负责人:
- 金额:$ 12.06万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-07-01 至 1993-06-30
- 项目状态:已结题
- 来源:
- 关键词:Dictyostelium cell differentiation cell motility chemotaxis complementary DNA developmental genetics endonuclease fungal genetics gel electrophoresis gene complementation gene expression gene mutation genes genetic manipulation genetic promoter element genetic regulation histogenesis molecular cloning molecular genetics mutagen testing nucleic acid biosynthesis nucleic acid hybridization nucleic acid sequence
项目摘要
The long range goal of this project is the application of molecular
genetic tools to understanding the cellular mechanisms and
processes that are necessary for differentiation and
morphogenesis. The objective is to identify, isolate and
characterize genes that are essential for development of
Dictoyostelium discoideum. This eucaryotic amoebal organism
provides a unique model system for the study of cellular functions
that are involved in development. During development, a uniform
population of amoebal cells differentiates into a multicellular
organism consisting of two cell types with a highly regulated
spatial organization. Motility, signal transduction, adhesion,
morphogenetic signalling and cell type specific gene regulation
are among the fundamental processes utilized by this organism to
accomplish its developmental program. The information gained
about how these functions are accomplished in Dictyostelium will
provide clues to similar functions in other organisms.
An indirect genetic approach to understanding the cellular
processes related to development will be taken. Mutants that
affect processes that are essential for development will be
identified, and then the genes that were mutated will be isolated.
In this way, a direct handle on critical functional elements can be
gained. Mutants that affect development are not new in
Dictyostelium, but the means to isolate the mutated genes has
never been available. The DNA mediated transformation system
will provide the means for both mutating and isolating these
genes. Two approaches to accomplishing this goal will be
undertaken in parallel. Antisense RNA synthesized from a
transformation vector will be used to inhibit expression of
essential developmental genes, or a vector that inserts into the
chromosome will be used to interrupt gene expression. In both
cases, large numbers of transformants will be screened for
defects in morphogenesis caused by the transformation vector.
The vector sequence will then provide the means for isolating the
inactivated gene. The purpose of this project is not simply to
clone more genes to study their regulation. Rather it is to use
functional criteria to isolate important genes as a means to
eventually studying the function of the gene products.
该项目的长期目标是分子生物学的应用
理解细胞机制的遗传工具
分化和分化所必需的过程
形态发生。 目的是识别、隔离和
描述对发育至关重要的基因
盘网网柄菌。 这种真核变形虫生物
为细胞功能的研究提供了独特的模型系统
参与开发的人员。 在开发过程中,统一
变形虫细胞群分化成多细胞
由两种细胞类型组成的生物体,具有高度调控
空间组织。 运动性、信号转导、粘附、
形态发生信号传导和细胞类型特异性基因调控
是该生物体利用的基本过程之一
完成其发展计划。 获得的信息
关于盘基网柄菌如何实现这些功能将
为其他生物体中的类似功能提供线索。
理解细胞的间接遗传学方法
将采取与开发相关的流程。 突变体
影响对发展至关重要的过程
鉴定出来,然后突变的基因就会被分离出来。
通过这种方式,可以直接处理关键功能元素
获得了。 影响发育的突变体在人类中并不新鲜。
盘基网柄菌,但分离突变基因的手段已经
从来没有可用过。 DNA介导的转化系统
将提供突变和隔离这些的方法
基因。 实现这一目标的两种方法是
并行进行。 反义RNA合成自
转化载体将用于抑制表达
必需的发育基因,或插入到的载体
染色体将被用来中断基因表达。 在两者中
情况下,将筛选大量转化体
由转化向量引起的形态发生缺陷。
然后,载体序列将提供分离
失活的基因。 这个项目的目的不仅仅是为了
克隆更多基因来研究它们的调控。 而是使用
分离重要基因的功能标准作为一种手段
最终研究基因产物的功能。
项目成果
期刊论文数量(0)
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{{ truncateString('DAVID A KNECHT', 18)}}的其他基金
MOLECULAR GENETICS OF DEVELOPMENT IN DICTYOSTELIUM
盘基网菌发育的分子遗传学
- 批准号:
3298321 - 财政年份:1988
- 资助金额:
$ 12.06万 - 项目类别:
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