AUTOCRINE/PARACRINE GROWTH FACTORS & LUNG MORPHOGENESIS

自分泌/旁分泌生长因子

• 批准号: 6128940
• 负责人: DAVID WARBURTON
• 金额: $ 36.79万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6128940
• 关键词: antisense nucleic acid; autocrine; biological signal transduction; cell cell interaction; cell differentiation; developmental genetics; embryo /fetus cell /tissue; fibroblast growth factor; genetic transcription; growth factor receptors; histogenesis; hormone regulation /control mechanism; immunoprecipitation; laboratory mouse; lung; mammalian embryology; organ culture; paracrine; protein binding; protein tyrosine kinase; receptor expression; respiratory epithelium

DESCRIPTION: (Adapted from the Investigator's Abstract) The Specific Aims are: 1)To determine the temporo-spatial localization of expression of mspry2 and to compare it with the respective temporo-spatial distribution of mspry1, 3, and 4 as well as with that of key FGF ligands (7 and 10), key FGF receptors (FGF-R2), and key effectors (phosphorylated MAPK) in early embryonic mouse lung morphogenesis; 2) To determine the respective functional specificity of mspry2&4 to negatively modulate mouse respiratory morphogenesis; 3) To determine whether mspry2 functions as an FGF ligand protein or binds candidate signaling proteins in the Ras pathway, and to determine which domains of mspry2 mediate these specific protein-protein functional interactions using mutational analysis; 4) To identify transcriptional control elements regulating spatial localization of mspry2 during lung branching morphogenesis.

描述：（改编自研究者摘要）具体目的是： 1）确定mspry 2表达的时空定位， 将其与mspry 1、3和4各自的时空分布进行比较 以及关键FGF配体（7和10），关键FGF受体（FGF-R2）， 和关键效应因子（磷酸化MAPK）在早期胚胎小鼠肺 形态发生; 2）确定 mspry 2和4负调节小鼠呼吸道形态发生; 3） 确定mspry 2是否作为FGF配体蛋白发挥功能或结合候选物 Ras通路中的信号蛋白，并确定mspry 2的哪些结构域 利用突变介导这些特定的蛋白质-蛋白质功能相互作用， 4）鉴定调控空间结构的转录调控元件 肺分支形态发生过程中mspry 2的定位。