AUTOCRINE/PARACRINE GROWTH FACTORS & LUNG MORPHOGENESIS

描述：(改编自《调查员摘要》)具体目标是： 1)确定mspry2和T0表达的时空定位 将其与mspry1、3和4各自的时空分布进行比较 以及关键的成纤维细胞生长因子配体(7和10)、关键的成纤维细胞生长因子受体(成纤维细胞生长因子-R2)、 和关键效应因子(磷酸化MAPK)在胚胎早期小鼠肺中的表达 形态发生；2)确定各自的功能特异性 Mspry2&4负性调控小鼠呼吸形态发生；3) 确定mspry2是作为成纤维细胞生长因子配体蛋白还是作为候选结合蛋白发挥作用 RAS途径中的信号蛋白，并确定mspry2的哪些结构域 利用突变基因介导这些特定的蛋白质-蛋白质功能相互作用 分析；4)确定转录调控元件调节空间 Mspry2在肺分支形态发生中的定位。

DESCRIPTION: (Adapted from the Investigator's Abstract) The Specific Aims are: 1)To determine the temporo-spatial localization of expression of mspry2 and to compare it with the respective temporo-spatial distribution of mspry1, 3, and 4 as well as with that of key FGF ligands (7 and 10), key FGF receptors (FGF-R2), and key effectors (phosphorylated MAPK) in early embryonic mouse lung morphogenesis; 2) To determine the respective functional specificity of mspry2&4 to negatively modulate mouse respiratory morphogenesis; 3) To determine whether mspry2 functions as an FGF ligand protein or binds candidate signaling proteins in the Ras pathway, and to determine which domains of mspry2 mediate these specific protein-protein functional interactions using mutational analysis; 4) To identify transcriptional control elements regulating spatial localization of mspry2 during lung branching morphogenesis.