Upconversion nanoparticle-based optical biosensor for early diagnosis of stroke

基于上转换纳米粒子的光学生物传感器用于中风的早期诊断

• 批准号: MR/Y503460/1
• 负责人: Sikha Saha
• 金额: $ 31.27万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FY503460%2F1
• 关键词: Upconversion; nanoparticle; based; optical; biosensor

Stroke is currently diagnosed by physical tests and studying images of brain scans to determine if the stroke has been caused by ischaemia (when a blockage cuts off the blood supply to the brain, e.g., blood clot) or a haemorrhage (blood vessel in the brain ruptures and causes bleeding inside the brain). Brain scans can reliably confirm bleeding, but many patients with suspected ischaemic stroke (up to 40%) are subsequently confirmed to have alternative pathologies e.g., migraine. seizures (stroke mimics) delaying transfer of stroke patients to acute stroke unit for early intervention and treatment. Thus, there is an urgent need for a biomarker for brain attack (stroke) just like there is one for heart attack. The current 'Gold Standard' technique for the detection of protein biomarkers is complex, time-consuming and requires automated equipment. Thus, it is important to develop and validate a rapid diagnostic tool for detection of blood biomarkers for stroke in the ambulance and Emergency Department, where a significant clinical problem lies i.e., the need to make a fastaccurate diagnosis of stroke. A simple and complimentary point of care (POC)biomarker test to differentiate stroke patients from non-stroke patients with similar clinical symptoms is essential in prehospital and emergency settings for efficient stroke management and early treatment. We have now developed a proof of principle biosensing tool for rapid analysis of potential biomarker in human blood serum. The proposed project focuses on the development and validation of a POC tool for early diagnosis of stroke-specific biomarkers to rapidly identify stroke patients. The POC biomarker assessment could be performed during initial triage avoiding delays in transporting stroke patients to appropriate care centres allowing expedited treatment to risk patients for better survival with improved quality of life. The proposed stroke of non stroke patients thus improving stroke patient flow and reducing expenses to the health care organisation.

目前，通过物理测试和研究脑扫描图像来诊断中风，以确定中风是由缺血引起的（当阻塞切断了对大脑的血液供应，例如血液凝块）或出血（脑部的血管中的血液血液凝块（脑中的血管）破裂并导致大脑内部流血）。脑部扫描可以可靠地证实出血，但是许多疑似缺血性中风（高达40％）的患者随后被确认具有替代性病理，例如偏头痛。癫痫发作（中风模拟）将中风患者转移到急性中风单位进行早期干预和治疗。因此，迫切需要生物标志物来进行脑攻击（中风），就像心脏病发作一样。当前检测蛋白质生物标志物的“金标准”技术是复杂的，耗时的，需要自动化设备。因此，重要的是要开发和验证快速的诊断工具，以检测救护车和急诊科中中风的血液生物标志物，在这种情况下，一个重大的临床问题在于，即需要快速诊断中风的诊断。一个简单而免费的护理点（POC）生物标志物测试，可将中风患者与具有相似临床症状的非中风患者区分开来，这对于院前和紧急情况至关重要，以进行有效的中风管理和早期治疗。现在，我们已经开发了一种原理生物传感工具的证明，用于快速分析人类血清中潜在的生物标志物。拟议的项目着重于对POC工具的开发和验证，用于早期诊断中风特异性生物标志物，以迅速识别中风患者。 POC生物标志物评估可以在初始分类过程中进行，避免将中风患者运送到适当的护理中心，从而使加快治疗的患者有风险以改善生存率，并改善生活质量。拟议的非中风患者中风，从而改善了中风患者的流量并减少了医疗保健组织的费用。