课题基金 / 基金详情

TARGETED DRUG RELEASE IN VIRUS-INFECTED MACROPHAGES

TARGETED DRUG RELEASE IN VIRUS-INFECTED MACROPHAGES
病毒感染的巨噬细胞中的靶向药物释放
批准号:
2784262
负责人:
Takuji Tsukamoto
金额:
$10.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-09-30 至 2001-03-31

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中文摘要
翻译
描述(改编自研究者摘要):研究者要求
英文摘要
DESCRIPTION (adapted from investigator's abstract): The investigators request funds for a Phase I SBIR to develop targeted delivery methods for anti-retroviral drugs to macrophages. Eventually, coated microparticles which contain lipid-bearing prodrugs will be ingested by macrophages in vitro, the microparticle coating will be hydrolytically unstable, and the lipid moieties will be linked via a HIV or CMV viral protease-digestible peptide linkage. The lipid moiety will be ceramide-based to facilitate subcellular localization of the lipid-linked prodrug to the virus-rich Golgi region within the macrophage. If the macrophages are infected with HIV or CMV, the respective viral proteases will cleave the lipid moiety from the prodrug and release the active form of the drug at the site of infection. The Phase I studies proposed in the present grant will use a model dye-peptide linkage and in vitro simulation. Release of the fluorescent dye from the microparticles following incubation with HIV or CMV proteases will provide proof of concept and pave the way for Phase II followup. Aim 1 will develop synthetic procedures for incorporation of viral protease-specific, cleavable bonds into drug attachment chemistries as well as HPLC procedures for analysis of the lipid-dye-complexes. Initially, ceramide-polypeptide(HIV)-dye and ceramide-polypeptide(CMV)-dye complexes will be prepared. Aim 2 will determine the rate of release of the model dye from the lipid-dye complex when incubated with HIV and CMV proteases. Recombinant HIV protease will be purchased, rhCMV protease will be provided by Dr. Scott Wong (ORPC) under subcontract. Both of these aims are preparatory to Aim 3 in which the lipid-dye-complexes will be used to coat microparticles of varying sizes. Finally, in Aim 4, the investigators will determine the best properties of the hydrolytically unstable coating for the microparticles to preclude premature dye release prior to macrophage ingestion. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE
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Fiber adsorbent for remediation of multisolute contamination in drinking water.
  • 批准号:
    9910154
  • 项目类别:
  • 资助金额:
    $15.9万
  • 财政年份:
    2020
  • 负责人:
    Takuji Tsukamoto
  • 依托单位:
Novel Fiber Scaffolding for Effective Removal of Diverse Hazardous Chemicals from Water
  • 批准号:
    9486775
  • 项目类别:
  • 资助金额:
    $0.25万
  • 财政年份:
    2016
  • 负责人:
    Takuji Tsukamoto
  • 依托单位:
Novel Fiber Scaffolding for Effective Removal of Diverse Hazardous Chemicals from Water
  • 批准号:
    9142176
  • 项目类别:
  • 资助金额:
    $15.0万
  • 财政年份:
    2016
  • 负责人:
    Takuji Tsukamoto
  • 依托单位:
Smart adsorption system for removal of toxic, organic chemicals from drinking wat
  • 批准号:
    8203614
  • 项目类别:
  • 资助金额:
    $15.0万
  • 财政年份:
    2011
  • 负责人:
    Takuji Tsukamoto
  • 依托单位:
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