UNIQUE, SMALL PARTICLE PHOSPHOR POWDERS FOR IMUNOASSAYS

用于免疫测定的独特小颗粒磷粉

• 批准号: 6015580
• 负责人: MARK J HAMPDEN-SMITH
• 金额: $ 8.07万
• 依托单位: SUPERIOR MICROPOWDERS, LLC
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-03 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6015580
• 关键词: diagnosis design /evaluation; immunologic assay /test; luminescence; method development; particle; phosphorescence; prostate specific antigen

Detection of histological markers for carcinomas has traditionally involved the staining of cells or cell markers with fluorescent dyes as indicators. However, autofluorescence is a common problem that ultimately limits the diagnostic utility of the results. This proposal attempts to overcome this limitation by the use of a new reporter technology called UPT (Up-Converting Phosphor Technology). This particle-based technology up-converts infrared to visible light which dose not exist in nature. Therefore, no background fluorescence is produced by the specimens being tested. Superior MicroPowders (SMP) teamed with STC Technologies proposes to develop new phosphor powders for use in phosphorescent immunoassays (PIAs). Superior MicroPowders will determine the feasibility of producing up-converter phosphor powders by spray routes to obtain powders with sizes in the range 400- 5nm. The luminescence and other materials characteristics of these powders will be analyzed to determine if they have suitable performance for use in PIAs. Candidate powders that meet these performance criteria will be used for screening in PIAs targeted at prostrate cancer. The availability of brighter phosphor powders with smaller particle size is expected to lead to broader applications including molecular diagnosis of cancer. The utility of up-converter phosphor powders will increase dramatically if they can be used as in situ probes for molecular detection by reducing their particle size below 400 nm. This proposal seeks to address this need by first concentrating their utility in a staining assay for PSA. If successful, future work will focus on the development of more colors of particles and detection of specific molecular markers.

癌的组织学标志物的检测传统上涉及用荧光染料作为指示剂对细胞或细胞标志物进行染色。然而，自体荧光是一个常见的问题，最终限制了结果的诊断效用。该提案试图通过使用称为UPT（上转换磷光体技术）的新报告技术来克服这种限制。这种基于粒子的技术将红外线向上转换为自然界中不存在的可见光。因此，待测样本不会产生背景荧光。上级微粉公司（SMP）与STC技术公司合作，提出开发用于磷光免疫测定（皮亚斯）的新荧光粉。上级微粉将决定通过喷雾路线生产上转换器磷光体粉末以获得尺寸在400- 5 nm范围内的粉末的可行性。将分析这些粉末的发光和其他材料特性，以确定它们是否具有用于皮亚斯的合适性能。符合这些性能标准的候选粉末将用于前列腺癌靶向皮亚斯的筛选。具有更小颗粒尺寸的更明亮的磷光体粉末的可用性预计将导致更广泛的应用，包括癌症的分子诊断。上转换荧光粉的效用将大大增加，如果他们可以作为原位探针分子检测，通过减少其粒径低于400 nm。该提案试图通过首先将其应用于PSA染色测定来解决这一需求。如果成功，未来的工作将集中在开发更多颜色的颗粒和检测特定的分子标记。