DEVELOPMENT OF ANTIVIRAL AGENTS AGAINST HIV1

全世界都在努力开发有效的抗病毒策略 抗HIV-1用于人类艾滋病的预防和干预 流行病。我们使用了两种方法来开发HIV-1抗病毒药物： 可将反义RNA转录为 抑制HIV-1复制；以及一种能够潜在地 产生免疫反应以预防艾滋病毒-1感染。体外研究 提示重组反义质粒DNA具有抑制HIV-1的作用 选择嗜T细胞和嗜巨噬细胞分离株的复制 表示发生在北美的病毒谱。当前 正在努力开发一种高效的包装细胞系统，用于 体内给药。对HIV-1疫苗DNA的研究表明， GAG和env基因在构建和发育过程中的作用 猴子的体液和细胞免疫反应。的安全评估 这两种抗病毒结构也将被用于评估潜在的 使用这些新方法的人类风险。 世界各地正在进行重大研究工作，以开发抗病毒药物 抗HIV-1用于人类艾滋病的预防和干预 流行病。反义DNA和质粒DNA疫苗领域是新的 作为潜在的治疗药物和疫苗被严格追逐 抗击艾滋病毒-1的战略。我们在这些领域的实验室研究准备 美国将积极提供相关产品的指导和专家审查 提交给FDA进行评估。

There is a world-wide effort to develop effective anti-viral strategies against HIV-1 for the prevention and intervention of the human AIDS epidemic. We have used two approaches to develop HIV-1 antivirals: a recombinant antisense plasmid DNA that can transcribe antisense RNAs to inhibit HIV-1 replication; and a plasmid DNA vaccine which can potentially generate immune responses to prevent HIV-1 infection. In vitro studies indicate that the recombinant antisense plasmid DNA can inhibit HIV-1 replication of selected T-cell-tropic and macrophage-tropic isolates that represent the spectrum of viruses occurring in North America. Current efforts are underway to develop an efficient packaging cell system for delivery in vivo. Studies of the HIV-1 vaccine DNAs indicate expression of the gag and env genes present in the construct and development of humoral and cellular immune responses in monkeys. Safety assessment of both antiviral constructs will be also be conducted to evaluate potential human risk using these novel approaches. Major research efforts are underway worldwide to develop antiviral agents against HIV-1 for the prevention and intervention of the human AIDS epidemic. The areas of antisense DNAs and plasmid DNA vaccines are novel and rigorously being pursued as potential therapeutics and vaccine strategies against HIV-1. Our laboratory research in these areas prepare us to actively provide guidance and expert review in related products submitted to the FDA for evaluation.