DEVELOPMENT OF A MONKEY MODEL FOR TESTING OF ANTIVIRAL AGENTS AGAINST HIV1

开发用于测试 HIV1 抗病毒药物的猴子模型

• 批准号: 6161245
• 负责人: Arifa S Khan
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161245
• 关键词: AIDS therapy; AIDS vaccines; Macaca nemestrina; antibody titering; disease /disorder model; human immunodeficiency virus 1; longitudinal animal study; model design /development; neutralizing antibody; nonhuman therapy evaluation; recombinant virus; simian immunodeficiency virus; virus DNA; virus infection mechanism; virus replication

Chimpanzees can be infected with HIV-1; however, since this species is endangered their maintenance cost is very high and the number of animals limited for experimental research. Thus a better model is sought. We have pursued HIV- 1 infection in pig-tailed macaques. Initially, we infected 2 monkeys with LAV/LAI, a laboratory strain of HIV-1 and 2 monkeys with a primary isolate of HIV-1. Analysis of the LAV-inoculated monkeys indicated that both monkeys were infected and contain viral sequences long-term. Current studies are underway to pursue host factors and viral determinants which control the establishment of infection, the level of virus production and the eventual progression to disease. The results from such studies will hopefully enable the pig-tailed monkeys to become a useful animal model for human AIDS. In addition, analysis of HIV-1 primary isolates in monkeys is also being carried out. Since the latter are representative of the natural infection in people, an animal model utilizing primary isolates will be valuable for the development and safety testing of effective HIV-1 vaccine(s) and therapeutic(s). There is a current epidemic of human AIDS. Major efforts and resources have been directed towards development of vaccines and therapeutics against HIV-1 and AIDS. The availability of a non-human primate model for AIDS is critical for evaluating the safety and efficacy of vaccines and therapeutics developed for human AIDS intervention and treatment. Furthermore, an animal model for HIV-1 infection will enable a better understanding of the mechanism of virus infection and development of disease. This information will provide better review of product submitted to FDA for evaluation.

黑猩猩可以感染HIV-1；然而，由于这种物种是 濒临灭绝的它们的维护成本非常高，动物的数量 有限用于实验研究。因此，人们寻求一种更好的模式。我们 已经在猪尾猕猴中追踪HIV-1感染。最初，我们 用HIV-1和HIV-2的实验室毒株LAV/LAI感染2只猴子 带有HIV-1主要分离株的猴子。三、分析了 接种LAV疫苗的猴子表明，这两只猴子都被感染了 长期含有病毒序列。目前的研究正在进行中，以进行 控制建立的宿主因素和病毒决定因素 感染、病毒产生水平和最终进展 为了疾病。这些研究的结果有望使 猪尾猴将成为人类艾滋病的有用动物模型。在……里面 此外，对猴子体内的HIV-1初级分离株的分析也在进行中 被执行。因为后者代表了自然的 在人类中感染，利用初级分离株的动物模型将是 对有效HIV-1的开发和安全性测试有价值 疫苗(S)和治疗性(S)。 目前有一种人类艾滋病的流行。主要工作和资源 一直致力于疫苗和疗法的开发 对抗HIV-1和艾滋病。非人灵长类动物模型的可用性 对于评估疫苗的安全性和有效性是至关重要的 以及为人类艾滋病干预和治疗开发的治疗学。 此外，HIV-1感染的动物模型将使更好的 对病毒感染机制及其发展的认识 疾病。此信息将为提交的产品提供更好的审查 提交给FDA进行评估。