NOVEL HUMAN BRONCHIAL EPITHELIAL CELL GENES INVOLVED IN AIRWAY INFLAMMATION

与气道炎症相关的新型人类支气管上皮细胞基因

• 批准号: 6161440
• 负责人: S LEVINE
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161440
• 关键词: antiinflammatory agents; cell adhesion molecules; colony stimulating factor; genetic regulation; human tissue; immunomodulators; inflammation; interleukin 10; molecular cloning; molecular genetics; neuropeptides; nitric oxide; nucleic acid sequence; polymerase chain reaction; respiratory epithelium; tissue /cell culture

Summary of Work: Human bronchial epithelial cells can initiate, amplify ,and attenuate airway inflammatory events via the production of pro- and anti-inflammatory molecules. Examples of pro-inflammatory human bronchial epithelial cell products include interleukins, chemokines, colony stimulating factors, lipid mediators, nitric oxide, adhesion molecules, and neuropeptides, while examples of anti-inflammatory products include soluble tumor necrosis factor receptors, interleukin-1 receptor antagonists, interleukin-10, and protease inhibitors. Since human airway epithelial cells participate in the regulation of inflammatory events, studies have been initiated to identify novel human airway epithelial cell genes that are involved in the regulation of airway inflammation. Utilizing differential display reverse transcriptase-polymerase chain reaction, candidate genes will be identified from a human pulmonary epithelial cell line following stimulation with either pro-inflammatory or anti-inflammatory mediators. Subsequent studies will utilize airway epithelial cells obtained from normal volunteers and volunteers with airway inflammatory disorders, such as asthma. Following the cloning and sequencing of candidate genes, studies will be undertaken to identify their function as well as factors regulating their expression. These studies will provide an increased understanding of the mechanisms by which human airway epithelial cells modulate inflammatory processes in the airway microenvironment. Furthermore, the identification of novel human airway epithelial cell genes may allow for the development of new therapeutic approaches for the reduction of airway inflammation in disorders such as asthma.

工作概述：人支气管上皮细胞具有初始化、扩增功能