GTP-BINDING PROTEINS AND SIGNAL TRANSDUCTION--STRUCTURE AND FUNCTION

GTP 结合蛋白和信号转导——结构和功能

• 批准号: 6162305
• 负责人: M RODBELL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6162305
• 关键词: G protein; adenylate cyclase; biological signal transduction; cell membrane; enzyme activity; immunocytochemistry; intracellular transport; lysosomes; muscarinic receptor; phosphoprotein phosphatase; protein kinase; protein structure function; protein transport; receptor binding; tissue /cell culture

Summary of Work: G-proteins have many properties suggestive of multimeric proteins such as actin and tubulin. Moreover, immunocytochemical analysis using imaging techniques (concoval microscopy) reveal punctate structures of Gs, Gi, and Go associated with the cytoskeletal network (particularly actin but also tubulin). Caveolae, invaginations of the surface membrane, have been investigated by purification of the membranes and immunochemical probes of the components. In NIH 3T3 cells, caveolae appear to contain G-proteins, but the purity of the structures remains uncertain. New purification procedures are being investigated along with efforts to further identify the punctate structures associated with the cytoskeletal network. The latter studies are being investigated with monoclonal antibodies linked to gold particles.

工作概述：G蛋白具有许多提示多聚体的性质。 蛋白质如肌动蛋白和微管蛋白。 此外，免疫细胞化学 使用成像技术（结膜显微镜）分析显示点状 与细胞骨架网络相关的Gs、Gi和Go结构 （特别是肌动蛋白，但也包括微管蛋白）。 小窝，内陷 表面膜，已经通过膜的纯化进行了研究 和组分的免疫化学探针。 在NIH 3T3细胞中，小窝 似乎含有G蛋白，但结构的纯度仍然存在 不确定 新的净化程序正在沿着研究中， 努力进一步确定点状结构相关的 细胞骨架网络 后一项研究正在进行调查， 单克隆抗体连接到金颗粒。