SIGNALING OF APOPTOSIS DURING CELL TRANSFORMATION

工作总结：正常的机体动态平衡是通过 仔细平衡细胞增殖和细胞死亡的过程。癌 细胞，在经历快速分裂和扩张的同时，也可能经历 细胞死亡。了解癌细胞如何死亡或丢失是很重要的。 它们对细胞死亡过程的敏感性。我们已经证明了 处于肿瘤过程不同阶段的细胞对 经历了不同的细胞凋亡。在单独的研究中，我们正在研究 氧化损伤反应中的细胞凋亡途径。在以下研究中 P53在这一过程中的作用，我们发现细胞凋亡也 似乎需要P85激酶。此外，我们还发现RB 蛋白质可能在细胞决定是否逮捕或 受伤后自杀。另外，我们也一直在研究， EIA结合的p300蛋白作为多个 细胞反应。我们发现p300可以与c-fos偶联。 在导致细胞凋亡的细胞压力条件下的蛋白质。这个 C-fos的升高已被证明是低血清诱导的一个关键事件 金黄地鼠成纤维细胞的凋亡。我们认为c-fos与 P300对于细胞决定经历细胞死亡至关重要。

Summary of Work: Normal organismal homeostasis is maintained through a careful balance of cell proliferation and cell death processes. Cancer cells, while rapidly undergoing division and expansion, may also undergo cell death. It is important to understand how cancer cells die or lose their susceptibility to the cell death process. We have shown that the susceptibility of cells at various stages of the neoplastic process to undergo apoptosis differs. In separate studies, we are pursuing the apoptotic pathway in response to oxidative damage. In studies following the role of p53 in this process, we have found that apoptosis also appears to require the p85 kinase. In addition we have found that the Rb protein may be a key player in the cellular decision whether to arrest or commit suicide following injury. In addition, we have also been examining the role of the EIA binding, p300 protein, as an integrator of multiple cellular responses. We have found that p300 can couple with the c-fos protein under conditions of cellular stress that result in apoptosis. The elevation of c-fos has been shown to be a key event in low serum induced apoptosis of hamster fibroblasts. We propose that the binding of c-fos to p300 is critical for the cellular decision to undergo cell death.