CARCINOGENIC INTERACTION OF RADIATION AND CHEMICALS

描述：BALB/c小鼠比C57 BL/6小鼠更易受 辐射引起的不稳定性，并假设这种差异 确定两种小鼠品系中的转化频率差异。 对F1后代的分析表明， 乳腺癌诱发是一种可遗传性状和初步数据 提示了一种类似的遗传模式， 诱导细胞遗传学不稳定性。 提出的实验将： 1)使用BALB/c和C57 BL/6小鼠的F1和F2子代来确定模式 对遗传不稳定性和辐射的易感性 转化; 2）使用这些重组近交系来检查 控制表型性状的基因座的共分离;和3）定位 决定易感性差异的基因座。 人们希望 这些基因座的定位将最终导致相关基因的克隆。 这些基因的鉴定应该有助于从机制上确定途径 参与诱导不稳定和转化， 有助于识别风险增加的人群亚群 辐射诱发的癌症

DESCRIPTION: BALB/c mice are more susceptible than C57BL/6 mice to radiation-induced instability and it is hypothesized that this difference determines transformation frequency differences in the two mouse strains. Analysis of F1 progeny suggests that the difference in susceptibility for induction of mammary cancer is a heritable trait and Preliminary Data suggests a similar pattern for inheritance of susceptibility to the induction of cytogenetic instability. Experiments are proposed which will: 1) use F1 and F2 progeny of BALB/c and C57BL/6 mice to determine the pattern of inheritance for susceptibility to genetic instability and radiation transformation; 2) use these recombinant inbred strains to examine co-segregation of the loci controlling the phenotypic traits; and 3) map the loci that determine the susceptibility differences. It is hoped that mapping of these loci will ultimately lead to cloning of the involved genes. Identification of such genes should help identify pathways mechanistically involved in the induction of instability and transformation and may be useful in identifying sub-populations of people who are at increased risk for radiation-induced cancer.