CORE--CELL CULTURE/TISSUE PREPARATION

核心——细胞培养/组织制备

• 批准号: 6345965
• 负责人: MARY L WILLIAMS
• 金额: $ 19.83万
• 依托单位: NORTHERN CALIFORNIA INSTITUTE/RES/EDU
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345965
• 关键词: biomedical facility; keratinocyte; tissue /cell culture

The Cell Culture Core supplies high quality cells for the Program Project investigators. The facility is maintained such that it meets and exceeds the relevant federal health and safety standards. The majority of cells provided by the facility are foreskin-derived normal human keratinocytes. These cells are plated with media, conditions, density etc. as required by each program project investigator. Presently the facility is providing over 250 million cells per week to investigators, but it can produce greater than or equal to 500 million per week on demand. In addition, the facility has a large library of transformed and genetically altered lines that are available to investigators. The most frequently used by the investigators are the squamous cell carcinoma lines (SCC-4, SCC-12F2, SCC-12B2); and low passage cells (HaCaT, A431). These are maintained at low levels which allows for rapid boosting of cell numbers at the investigator's request. Dr. Mary L. Williams, Director, & Ms. Sally Pennypacker, Heady Technician, not only provide in vitro culture knowledge and expertise for other investigators, but also insure that health and safety inspections are passed; hoods and incubators are tested and serviced regularly; and that cultures are tested monthly for mycoplasma. Both Dr. Williams & Ms. Pennypacker also possess expertise in culturing basal cell carcinoma cells, adult skin keratinocytes and fibroblasts, fetal skin keratinocytes, late passage keratinocytes, fetal mouse skin keratinocytes; newborn and adult mouse keratinocytes; lifted keratinocyte cultures; and proficiency in explant methods. Core A also is developing fibroblast-free fetal keratinocytes, and optimizing both BCC isolation and late-passage keratinocyte cultures (i.e., greater pass 25). A fundamental part of the operation of Core A is the interaction between its personnel and members of the Program Project, allowing researchers to obtain high-quality cultures specifically tailored to their needs, while providing direct feedback for optimizing of techniques and protocols. The facility is now equipped with amber lights for work with light-sensitive compounds (e.g., retinoids, vitamin D and derivatives). Finally, Core A provides both maximum numbers of high quality cells and advice on in vitro methods to investigators, who rely heavily on this facility. The continued production of healthy keratinocytes will remain the priority of this key facility.

The Cell Culture Core supplies high quality cells for the Program Project investigators. The facility is maintained such that it meets and exceeds the relevant federal health and safety standards.该设施提供的大多数细胞是包皮来源的正常人类角质形成细胞。 These cells are plated with media, conditions, density etc. as required by each program project investigator. Presently the facility is providing over 250 million cells per week to investigators, but it can produce greater than or equal to 500 million per week on demand.此外，该设施还拥有一个大型的转化和转基因品系库可供研究人员使用。研究人员最常用的是鳞状细胞癌细胞系（SCC-4、SCC-12F2、SCC-12B2）；和低传代细胞（HaCaT，A431）。这些保持在较低水平，以便根据研究人员的要求快速增加细胞数量。 Dr. Mary L. Williams, Director, & Ms. Sally Pennypacker, Heady Technician, not only provide in vitro culture knowledge and expertise for other investigators, but also insure that health and safety inspections are passed; hoods and incubators are tested and serviced regularly; and that cultures are tested monthly for mycoplasma. Both Dr. Williams & Ms. Pennypacker also possess expertise in culturing basal cell carcinoma cells, adult skin keratinocytes and fibroblasts, fetal skin keratinocytes, late passage keratinocytes, fetal mouse skin keratinocytes;新生和成年小鼠角质形成细胞；提升角质形成细胞培养物；并熟练掌握外植体方法。 Core A also is developing fibroblast-free fetal keratinocytes, and optimizing both BCC isolation and late-passage keratinocyte cultures (i.e., greater pass 25). A fundamental part of the operation of Core A is the interaction between its personnel and members of the Program Project, allowing researchers to obtain high-quality cultures specifically tailored to their needs, while providing direct feedback for optimizing of techniques and protocols.该设施现在配备了琥珀色灯，用于处理光敏化合物（例如类维生素A、维生素 D 和衍生物）。 Finally, Core A provides both maximum numbers of high quality cells and advice on in vitro methods to investigators, who rely heavily on this facility. The continued production of healthy keratinocytes will remain the priority of this key facility.