TISSUE SPECIFIC CNTRL ALPHA 1B ANDRENOCEPTOR EXPRESSION

组织特异性 CNTRL ALPHA 1B 雄激素受体表达

• 批准号: 6172833
• 负责人: bin gao
• 金额: $ 9.9万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-03 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6172833
• 关键词: DNA footprinting; affinity chromatography; alpha adrenergic receptor; binding proteins; cell type; cofactor; gel mobility shift assay; genetic promoter element; heart cell; laboratory rat; liver cells; neoplastic cell; receptor expression; tissue /cell culture; transcription factor; transfection; yeast two hybrid system

DESCRIPTION: The alpha1B adrenergic receptor (alpha1BAR) is a G-protein-coupled receptor that plays an important role in the control of cardiovascular homeostasis and metabolic processes in the liver, and recent studies have shown differential regulation of the transcription of the alpha1BAR gene in heart and liver under a variety of conditions. Dr. Gao will test the hypothesis that this differential regulation may be due to the tissue specific modulation of the action of the same transcription factor or to the interaction of the gene with different, tissue-specific transcription factors. Nuclear factor 1 (NF1) activates transcription driven by the dominant P2 promoter of the alpha1BAR gene in Hep3B cells and primary hepatocytes, but inhibits it in DDT1MF-2 cells. This duality may be due to modulation of the activity of NF1 by cell-specific cofactors. These cofactors will be identified by using the yeast two-hybrid system. The tissue distribution of NF1 parallels the relative abundance of the 2.7-kb alpha1BAR mRNA species, except for the heart, which expresses high levels of alpha1BAR and its 2.7 kb mRNA but little, if any, NFl. This suggests that transcription of the alpha1BAR gene in heart involves factor(s) other than NF1. Indeed, DNase I footprinting and DNA gel mobility shift analyses showed that the region -721 to -723 within the P2 promoter binds a new, previously unidentified transcription factor, called HF, in heart. This factor will be further characterized, purified by DNA affinity chromatography and cloned, if necessary. The role of this factor in transcription control will be identified by mutational and transfection analyses. Identification of the cell type-specific cofactors involved in modulating function of NF1 and the cardiac-specific transcription factors involved in the transcription of alpha1BAR gene in heart will shed light on the molecular mechanisms involved in the tissue specific regulation of alpha1BAR gene expression.

描述：alpha1B肾上腺素能受体（alpha1BAR）是一种

项目成果

DNase I footprinting analysis of transcription factors recognizing adrenergic receptor gene promoter sequences.

识别肾上腺素受体基因启动子序列的转录因子的 DNase I 足迹分析。

• DOI: 10.1385/1-59259-684-3:419
• 发表时间: 2000
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Gao,B;Kunos,G
• 通讯作者: Kunos,G

The simian virus 40 core C enhancer-like element is a positive regulator in the rat alpha1B adrenergic receptor gene proximal promoter.

猿猴病毒40核心C增强子样元件是大鼠α1B肾上腺素受体基因近端启动子的正调节因子。

• DOI: 10.1006/bbrc.1998.9860
• 发表时间: 1998
• 期刊: Biochemical and biophysical research communications.
• 作者: Dao,N;Gao,B
• 通讯作者: Gao,B

Southwestern blots for detection of a DNA binding protein recognizing the alpha 1B-adrenergic receptor gene promoter.

用于检测识别 α1B-肾上腺素能受体基因启动子的 DNA 结合蛋白的西南印迹。

• DOI: 10.1385/1-59259-684-3:411
• 发表时间: 2000
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Gao,B;Kunos,G
• 通讯作者: Kunos,G