CALCIUM DEPENDENT MECHANISMS IN APOPTOSIS

描述(改编自调查员摘要)：当PI和其他人 已经证明了改变细胞内的需要 钙水平在调节细胞凋亡中的作用，可获得的数据很少 关于这一因子的细胞内靶点。受钙调控的A组 蛋白水解酶已经通过PI从细胞核中鉴定出来，似乎是 这是层粘连蛋白B降解和DNA断裂所必需的。少年派有 也证明了在Caspase中存在一个共同的切割序列 IP3受体。该建议旨在：1)确定 胸腺细胞凋亡所需的钙激活的蛋白酶；2)确定 抗凋亡蛋白bcl-2调节这种蛋白水解酶，并决定3) 如果细胞凋亡需要1型IP3受体。

DESCRIPTION (adapted from investigator's abstract): While the PI and others in the field have demonstrated a requirement for altered intracellular calcium levels in the regulation of apoptosis, little data are available regarding the intracellular targets for this factor. A calcium regulated protease has been identified by the PI from the nucleus that appears to be required for both lamin B degradation and DNA fragmentation. The PI has also demonstrated that there is a caspase cleavage consensus sequence in the IP3 receptor. This proposal is designed to: 1) identify the calcium-activated protease required for thymocyte apoptosis; 2) determine if the anti-apoptotic protein bcl-2 regulates this proteases and 3) determine if the type 1 IP3 receptor is required for apoptosis.