CALCIUM DEPENDENT MECHANISMS IN APOPTOSIS

描述（改编自研究者摘要）：PI和其他人 已经证明需要改变细胞内 钙水平在细胞凋亡的调节，很少有数据可用 关于这个因子的细胞内靶点。 钙调节 蛋白酶已经被PI从细胞核中鉴定出来， 核纤层蛋白B降解和DNA片段化都需要。 主要研究者有 也证明了在细胞中存在半胱天冬酶切割共有序列， IP 3受体。 该建议旨在：1）确定 胸腺细胞凋亡所需的钙激活蛋白酶; 2）确定是否 抗凋亡蛋白bcl-2调节这种蛋白酶，以及3）确定 如果1型IP 3受体是凋亡所必需的。

DESCRIPTION (adapted from investigator's abstract): While the PI and others in the field have demonstrated a requirement for altered intracellular calcium levels in the regulation of apoptosis, little data are available regarding the intracellular targets for this factor. A calcium regulated protease has been identified by the PI from the nucleus that appears to be required for both lamin B degradation and DNA fragmentation. The PI has also demonstrated that there is a caspase cleavage consensus sequence in the IP3 receptor. This proposal is designed to: 1) identify the calcium-activated protease required for thymocyte apoptosis; 2) determine if the anti-apoptotic protein bcl-2 regulates this proteases and 3) determine if the type 1 IP3 receptor is required for apoptosis.