AN ANIMAL MODEL FOR STUDYING TMJ INFLAMMATION IN RATS

研究大鼠颞下颌关节炎症的动物模型

• 批准号: 6205417
• 负责人: LARRY L BELLINGER
• 金额: $ 2.75万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205417
• 关键词: Freund's adjuvant; analgesics; antiinflammatory agents; biomarker; calcitonin gene related peptide; computer simulation; corticosterone; disease /disorder model; endorphins; inflammation; interleukin 1; laboratory rat; mechanical stress; model design /development; molecular dynamics; nutrient intake activity; oxytocin; pathologic process; prognosis; prostaglandin E; substance P; temporomandibular joint syndrome

DESCRIPTION: (Adapted from Abstract) The primary objective of this proposal is to develop a biobehavioral animal model that will allow for better design of experiments investigating TMJ inflammation. The working hypothesis is that animal feeding patterns will change and will be directly correlated with biological markers during complete Freud's adjuvant (CFA) induced TMJ inflammation. Feeding behavior will be determined via a sophisticated and highly sensitive method of computer operated meal pattern analyses (MPA). In the first experiment, a generalized model will be further developed that uses MPA to determine behavioral changes during early (+2 days after CFA) and late (+42 days after CFA) stage TMJ inflammation. Meal patterns will be correlated to changes in inflammatory markers including histology, TMJ calcitonin gene related peptide and interleukin-1b, trigeminal ganglia substance P, and plasma stress hormones corticosterone, b-endorphin and oxytocin. A second experiment will repeat these procedures with and without anti-inflammatory treatment. These experiments are intended to provide the clinician with a non- invasive behavioral marker for TMJ inflammation and stress, and a more objective basis for patient diagnosis, treatment and outcomes assessment.

描述：（改编自摘要）本提案的主要目标是 开发一种生物行为动物模型， 研究颞下颌关节炎症的实验。工作假设是， 动物饲养模式将发生变化， 完全弗氏佐剂（CFA）诱导TMJ过程中的生物学标志物 炎症喂食行为将通过一个复杂的， 高度灵敏的计算机操作的膳食模式分析（MPA）方法。在 在第一个实验中，将进一步开发一个通用模型， MPA确定早期（CFA后+2天）和晚期的行为变化 （CFA后+42天）TMJ炎症阶段。饮食模式将与 炎症标志物的变化，包括组织学，TMJ降钙素基因 相关肽和白细胞介素-1b、三叉神经节P物质和血浆 应激激素皮质酮，b-内啡肽和催产素。第二个实验 将重复这些程序与和没有抗炎治疗。 这些实验旨在为临床医生提供非侵入性的 为颞下颌关节炎症和应激的行为学标记，提供了更客观的依据 用于患者诊断、治疗和结果评估。