AN ANIMAL MODEL FOR STUDYING TMJ INFLAMMATION IN RATS

描述：(摘自摘要)本提案的主要目标是 开发一种生物行为动物模型，以便更好地设计 研究TMJ炎症的实验。工作假说是 动物的饲养模式将发生变化，并将直接与 完全弗洛伊德佐剂诱导TMJ过程中的生物标志物 发炎。摄食行为将通过复杂的 计算机操作进餐模式分析的高灵敏方法(MPA)。在……里面 第一个实验将进一步开发一个通用模型，该模型使用 确定早期(CFA后+2天)和后期的行为变化 (CFA后+42天)TMJ炎症分期。餐饮模式将相互关联 组织学、TMJ降钙素基因等炎症标志物的变化 相关肽和白介素1b、三叉神经节P物质和血浆 应激激素皮质酮、β-内啡肽和催产素。第二次实验 在接受和不接受抗炎治疗的情况下，将重复这些程序。 这些实验旨在为临床医生提供一种非侵入性 TMJ炎症和应激的行为标志物，以及更客观的基础 用于患者诊断、治疗和结果评估。

DESCRIPTION: (Adapted from Abstract) The primary objective of this proposal is to develop a biobehavioral animal model that will allow for better design of experiments investigating TMJ inflammation. The working hypothesis is that animal feeding patterns will change and will be directly correlated with biological markers during complete Freud's adjuvant (CFA) induced TMJ inflammation. Feeding behavior will be determined via a sophisticated and highly sensitive method of computer operated meal pattern analyses (MPA). In the first experiment, a generalized model will be further developed that uses MPA to determine behavioral changes during early (+2 days after CFA) and late (+42 days after CFA) stage TMJ inflammation. Meal patterns will be correlated to changes in inflammatory markers including histology, TMJ calcitonin gene related peptide and interleukin-1b, trigeminal ganglia substance P, and plasma stress hormones corticosterone, b-endorphin and oxytocin. A second experiment will repeat these procedures with and without anti-inflammatory treatment. These experiments are intended to provide the clinician with a non- invasive behavioral marker for TMJ inflammation and stress, and a more objective basis for patient diagnosis, treatment and outcomes assessment.