ULTRASOUND-INDUCED HYPERTHERMIC TERATOGENICITY

超声诱导的高温致畸性

• 批准号: 6182487
• 负责人: Morton Miller
• 金额: $ 7.95万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6182487
• 关键词: bioimaging /biomedical imaging; brain disorders; embryo /fetus; gestational age; heat injury; hyperthermia; laboratory rat; neural plate /tube; prenatal growth disorder; radiation dosage; teratogens; tissue /cell culture; ultrasound; ultrasound biological effect

DESCRIPTION: (Adapted from applicant's abstract) This proposal is designed to test the general hypothesis that fetal ultrasound exposure at clinically relevant frequencies, intensities, and dwell times is capable of heating the rat fetus by several degrees Celsius, and that this heating, if appropriately timed, is sufficient to cause neural tube defects. It initially examines the thermal environment of the pregnant rat and re-examines the issue of the teratogenic effect of maternal heating by immersion to serve as a positive control. Then it will determine the ultrasound dosimetry necessary to raise fetal temperature 5, 3, and 1 degree Celsius in an acute preparation followed by a randomized dosing sequence designed to raise fetal temperature by those amounts for 1, 5, or 10 minutes to evaluate teratogenic effect. Finally, a series of in vitro experiments using rat sarcoma cells and a clonogenic assay will be used to determine the cell killing effects of each of these thermal exposures.

描述：（改编自申请人的摘要）该建议旨在 测试临床上胎儿超声暴露的一般假设 相关频率，强度和停留时间能够加热 大鼠胎儿几摄氏度，如果适当的话，这种加热 定时，足以引起神经管缺陷。它最初检查了 怀孕大鼠的热环境和重新检查 浸入孕产妇加热的致畸作用，作为阳性 控制。然后，它将确定升高所需的超声剂量法 急性制剂中的胎儿温度5、3和1度摄氏 通过随机给药序列，旨在提高胎儿温度 1、5或10分钟的量以评估致畸作用。最后，一个 使用大鼠肉瘤细胞和克隆原料测定的一系列体外实验 将用于确定这些热的每一个的细胞杀死效应 暴露。

项目成果

Arrhenius thermodynamics and birth defects: chemical teratogen synergy. Untested, testable, and projected relevance.

阿累尼乌斯热力学和出生缺陷：化学致畸剂的协同作用。

• DOI: 10.1002/bdrc.21025
• 发表时间: 2013
• 期刊: Birth defects research. Part C, Embryo today : reviews
• 作者: Miller,MortonW;Church,CharlesC
• 通讯作者: Church,CharlesC